Contact

Dr. Margitta Dathe

phone +49 30 94793 274
fax +49 30 94793 269
dathe(at)fmp-berlin.de

 

 

Peptide-Lipid Interaction (Margitta Dathe)

We have been interested in the interaction of cell-penetrating and cell-permeabilizing peptides with membranes of human and bacterial cells. We exploit membrane-translocating peptides as targeting and uptake-mediating tools for lipid-based carrier systems loaded with diagnostic or therapeutic drug molecules. Recent efforts have been focused on targeting the blood-brain barrier and delivering bioactive compounds into the skin. Furthermore, we have elucidated the mode of antimicrobial action of small cyclic arginine and tryptophan-rich antimicrobial peptides and try currently to get insight into the activity spectrum of cyclic peptides against fungi and eukaryotic cells.
The methods we have mainly been using are fluorescence-based approaches on the cellular level such as confocal laser scanning microscopy (CLSM) and fluorescence-activated cell sorting (FACS) analysis. The characterization of lipid-based carriers and of peptide interactions with membrane-modelling lipid systems includes biophysical methods such as light scattering, fluorescence and surface potential, microcalorimetry, and chromatography. These analytical techniques are complemented by toxicity studies in relevant mammalian cells and antimicrobial assays with various bacterial strains.

Leibniz-Forschungsinstitut für Molekulare Pharmakologie im Forschungsverbund Berlin e.V. (FMP)
Campus Berlin-Buch
Robert-Roessle-Str. 10
13125 Berlin, Germany
+4930 94793 - 100 
+4930 94793 - 109 (Fax)
info(at)fmp-berlin.de