Contact

Dr. Margitta Dathe

phone +49 30 94793 274
fax +49 30 94793 269
dathe(at)fmp-berlin.de

 

 

Peptide-Lipid Interaction / Peptide Transport (Margitta Dathe)

Our group is interested in the selective interaction of peptides with cellular membranes and their ability to penetrate or permeabilize them. We exploit these properties in the development of peptides as targeting and uptake-mediating tools for drugs and lipid-based drug carriers. Currently, our activities focus on targeting the blood-brain barrier and the efficient cutaneous delivery of bioactive compounds. Furthermore, we are attempting to elucidate the mode of action of small cyclic membrane active antimicrobial peptides and to optimize their activity for selected applications.
The methods we mainly use to tackle the research projects are fluorescence-based approaches on the cellular level such as confocal laser scanning microscopy (CLSM) and fluorescence-activated cell sorting (FACS) analysis. The characterization of lipid-based carriers and of peptide interactions with membrane-modelling lipid systems includes biophysical methods such as light scattering, fluorescence and surface potential and electrophysiological measurements, microcalorimetry, and chromatography. These analytical techniques are complemented by toxicity studies in relevant mammalian cells and antimicrobial assays with various bacterial strains.

Leibniz-Forschungsinstitut für Molekulare Pharmakologie im Forschungsverbund Berlin e.V. (FMP)
Campus Berlin-Buch
Robert-Roessle-Str. 10
13125 Berlin, Germany
+4930 94793 - 100 
+4930 94793 - 109 (Fax)
info(at)fmp-berlin.de