Department of NMR-Supported Structural Biology
(Hartmut Oschkinat)

Prof. Dr. Hartmut Oschkinat

Leibniz-Institut für Molekulare Pharmakologie (FMP)  
Department NMR-supported Structural Biology
  
Robert-Rössle-Str. 10 / D 13125 Berlin
Phone: +49 (0)30 / 94793 160
Fax: +49 (0)30 / 94793 169
  
E-mail: oschkinat@fmp-berlin.de

 

Research Interests and Achievements

Structural characterisation of protein-protein interactions responsible for the reception and transduction of signals in biological systems, and their disruption by small molecules. Protein domains which recognize peptides with characteristic motifs, proteins involved in protein homeosthasis, structural investigations on membrane proteins and cytoskeleton-attached proteins by solid state NMR.
More than 180 publications in the fields of protein structure and NMR methods development.  More than 50 structures deposited in the PDB. Among those are first structures of the PH- and WW-domains. Development of small molecular inhibitors of interactions of PDZ domains. First assignment of protein resonances on the basis of solid-state NMR data, first structure of a protein by solid-state NMR. Structure of a fibril formed by a WW-domain at atomic resolution. Structure of alphaB-crystallin oligomers by solid-state NMR and SAXS. Applications of dynamic nuclear polarisation to large biological complexes. Structure of the dark-adapted form of bacteriorhodopsin by solution NMR.

 

Curriculum Vitae

2009-2011

Acting Director of the Leibniz-Institut für Molekulare Pharmakology (FMP), Berlin
Since
1998  
Head of the department "NMR-supported Structural Biology“ at the 
Leibniz-Institut für Molekulare Pharmakologie, Professor of Structural Chemistry at the Free University in Berlin
1992-1998Group leader at the EMBL, Heidelberg. Work on structure and function of signalling domains
1992Habilitation in Biophysical Chemistry at the Technical University of Munich
1987-1991  Position as NMR-spectroscopist at the Max-Planck-Institute for Biochemistry (Martinsried, Germany), first in the Clore/Gronenborn group, later independently in the department of Prof. Huber. Pulse sequence development, 3D-NMR
1986-1987Postdoctoral work with Prof. Dr. Bodenhausen at the University of
Lausanne, Switzerland; work on pulse sequences
1986Graduate thesis: "Analysis of the conformation of Cyclosporin in solution using NMR-spectroscopy: development and use of new methods"
1983-1985Completion of dissertation in Prof. Kessler's Laboratory at the University of Frankfurt
1983-1984Visit to the laboratory of Prof. Dr. Ray Freeman, Oxford, England
1975-1976
and 1978-1983
Chemistry degree at the University of Frankfurt, with merit; Diploma (excellent)

 

Activities in the scientific community

1998  EMBO-Membership


Editorial Boards

Journal of Biomolecular NMR, Springer Netherlands
Structure, Elsevier, San Diego
Journal of Structural Genomics, Springer Netherlands

 

Advisory Boards

CEITEC, Brno, Czech Republic
CCPN, Cambridge, UK
 

Selected Publications

Jehle S, Rajagopal P, Bardiaux B, Markovic S, Kühne R, Stout JR. Higman VA, Klevit RE, van Rossum BJ, Oschkinat H (2010) Solid-state NMR and SAXS studies provide a structural basis for the activation of aB-crystallin oligomers. Nat Struct Mol Biol, 17: 1037-1042.

Krabben Lvan Rossum BJ, Jehle S, Bocharov E, Lyukmanova EN, Schulga AA, Arseniev A, Hucho F, Oschkinat H (2009) Loop 3 of short neurotoxin II is an additional interaction site with membrane-bound nicotinic acetylcholine receptor as detected by solid-state NMR spectroscopy. J Mol Biol, 390:662-671.

Pellecchia M, Bertini I, Cowburn D, Dalvit C, Giralt E, Jahnke W, James TL, Homans SW, Kessler H, Luchinat C, Meyer B, Oschkinat H, Peng J, Schwalbe H, Siegal G (2008) Perspectives on NMR in drug discovery: a technique comes of age. Nat Rev Drug Discov, 7(9):738-745.

Ferguson N, Becker J, Tidow H, Tremmel S, Sharpe TD, Krause G, Flinders J,  Petrovich M, Berriman J, Oschkinat H, Fersht AR (2006) General structural motifs of amyloid protofilaments. Proc Natl Acad Sci USA 103:16248-16253.

Joshi M, Vargas C, Boisguerin P, Diehl A, Krause G, Schmieder P, Moelling K, Hagen V, Schade M, Oschkinat H. 2006. Discovery of low-molecular-weight ligands for the AF6 PDZ domain. Angew Chem Int Ed Engl. 45(23):3790-3795.

Castellani F, van Rossum BJ, Diehl A, Schubert M, Rehbein K, Oschkinat H (2002) Structure of a protein determined by solid-state magic-angle-spinning NMR spectroscopy. Nature 420:98-102.

Macias MJ, Gervais V, Civera C, Oschkinat H (2000) Structural analysis of WW domains and design of a WW prototype. Nat Struc Biol 7:375-79.

Macias MJ, Hyvˆnen M, Baraldi E, Schultz J, Sudol M, Saraste M, Oschkinat H (1996) Structure of the WW domain of a kinase-associated protein complexed with a proline-rich peptide. Nature 382:646-9.

Macias-Hernandez M, Musacchio A, Ponstingl H, Nilges M, Saraste M, Oschkinat H (1994) Structure of the pleckstrin homology domain from b-spectrin. Nature 369:675-7.

Oschkinat H, Griesinger C, Kraulis PJ, SÀrensen OW, Ernst RR, Gronenborn AM, Clore GM (1988) Three-dimensional NMR-spectroscopy of a protein in solution. Nature 332:374-6. 

Leibniz-Forschungsinstitut für Molekulare Pharmakologie im Forschungsverbund Berlin e.V. (FMP)
Campus Berlin-Buch
Robert-Roessle-Str. 10
13125 Berlin, Germany
+4930 94793 - 100 
+4930 94793 - 109 (Fax)
info(at)fmp-berlin.de