Core Facility

Martin Lehmann

Cellular Imaging Facility

Martin Lehmann Portrait

We address questions of biological and pharmacological importance using light microscopy techniques (confocal laser scanning microscopy, two-photon excitation, uncaging processes, FCS, FRAP, FRET, FLIM, cellular uptake of peptides, colocalization studies, intracellular ion concentrations, protein translations studies, etc.) in conjunction with the electron microscopic and electrophysiological techniques.


Services

The Cellular Imaging Facility assists the users at every stage of their imaging projects, from project design, microscopy training, sample preparations, assistance in imaging to image data analysis and visualization.

Light Microscopy 

The Light Microscopy Unit supports the researchers with high-end fluorescence imaging technology to study living cells, microorganisms, and tissue organization. We aid the study of proteins and membranes at the nanoscale using our super-resolution and TIRF microscopes. We also assist in single-cell and molecular imaging techniques such as FRET, FRAP, FLIM, TIRFM, FCS, ion measurements, and caged compounds.

Electron Microscopy 

The Electron Microscopy Unit provides support in the visualization of cellular ultrastructure and in localizing individual proteins at the subcellular level. The EM unit also supports the users with sample preparation techniques, immunogold labeling, correlative light and electron microscopy (CLEM), 3D electron microscopy using tomograpical reconstruction or Focused Ion Beam Scanning Electron microsopy (FIB-SEM), and image data analysis.

Image Data Analysis

The Image Analyst in our facility provide consultation and training in image analysis methods and techniques for extracting meaningful quantitative measurements from images. We also support developing custom automated workflows for image data analysis and visualization. Support with image analysis software such as FIJI/ImageJ, Huygens, Imaris is also available. Every two weeks "Image Analysis Table Talk" is offered which is free for all users to come by and talk about image analysis projects and get help. 

Contact


Research Section

Molecular Physiology & Cell Biology

Equipment


LSM780

  • Fluorescence channels: 34-Channel QUASAR Detection Unit
  • Laser: 405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 594 nm, 633 nm
  • Definite Focus
  • Heating table unit

LSM710-ConfoCor3

  • Fluorescence channels: 34-Channel QUASAR Detection Unit
  • Laser: 405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 633 nm
  • Equipped with a FCS-setup (Fluorescence Correlation Spectroscopy)
  • Definite Focus
  • Heating table unit

LSM510-META-NLO

  • Fluorescence channels: 3 - one is a META detector
  • Laser: 458 nm, 488 nm, 514 nm, 543 nm, 633 nm and a tunable IR-laser
  • Multiphoton excitation: 720 - 930 nm
  • Equipped with a FLIM-setup (Becker & Hickl GmbH)
  • Heating table unit

LSM780

  • Fluorescence channels: 34-Channel QUASAR Detection Unit
  • Laser: 405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 594 nm, 633 nm
  • Definite Focus
  • Heating table unit

LSM710-ConfoCor3

  • Fluorescence channels: 34-Channel QUASAR Detection Unit
  • Laser: 405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 633 nm
  • Equipped with a FCS-setup (Fluorescence Correlation Spectroscopy)
  • Definite Focus
  • Heating table unit

LSM510-META-NLO

  • Fluorescence channels: 3 - one is a META detector
  • Laser: 458 nm, 488 nm, 514 nm, 543 nm, 633 nm and a tunable IR-laser
  • Multiphoton excitation: 720 - 930 nm
  • Equipped with a FLIM-setup (Becker & Hickl GmbH)
  • Heating table unit

ZEISS-CSU

  • Perkin Elmer UltraView
  • Yokogawa Spinning disc
  • EMCCD Camera
  • Laser: 405 nm, 488 nm, 568 nm, 640 nm
  • Piezo Z-drive for fast 3D Imaging
  • FRAP unit
  • Velocity Software and analysis

Nikon-CSU

  • Nikon Ti Eclipse with Perfect Focus System and automatic stage
  • Yokogawa Spinning disc (CSU-X1)
  • EMCCD Camera (Andor AU-888)
  • Laser: 405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 638 nm
  • Fast triggered aquisition
  • Piezo Z-drive for fast 3D imaging
  • FRAPPA and 16-Color LED for FRAP and fast optogenetics
  • Incubator with temperature and CO2 control (OKOLAB)
  • NIS-Elements for control and analysi
Nikon CSU

ZEISS-CSU

  • Perkin Elmer UltraView
  • Yokogawa Spinning disc
  • EMCCD Camera
  • Laser: 405 nm, 488 nm, 568 nm, 640 nm
  • Piezo Z-drive for fast 3D Imaging
  • FRAP unit
  • Velocity Software and analysis

Nikon-CSU

  • Nikon Ti Eclipse with Perfect Focus System and automatic stage
  • Yokogawa Spinning disc (CSU-X1)
  • EMCCD Camera (Andor AU-888)
  • Laser: 405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 638 nm
  • Fast triggered aquisition
  • Piezo Z-drive for fast 3D imaging
  • FRAPPA and 16-Color LED for FRAP and fast optogenetics
  • Incubator with temperature and CO2 control (OKOLAB)
  • NIS-Elements for control and analysi
    Nikon CSU

gated STED (Stimulated Emission Depletion)

  • Leica TCS SP8 3X
  • 5 color gated STED with ~40-60 nm lateral resolution
  • 3D STED with ~130 nm isotropic resolution
  • Fluorescence channels: 2 x PMT, 2 x HyD Sp GaAsP
  • Laser: tunable WLL 470-670 nm, 405 nm diode laser
  • Depletion laser (STED): 592 nm, 775 nm
  • Fluorescence lifetime upgrade: Falcon & tauSTED
  • Leica Las X & Huygens Deconvolution

STORM/PALM/STEDYCON

  • Nikon Ti Eclipse with Perfect Focus System
  • Multicolor dSTORM/single particle tracking with 30 nm resolution
  • 3D STORM with 25 nm lateral and 60 nm axial resolution
  • Optosplit II and EMCCD (Andor Ultra 897) for fast multicolor imaging
  • Laser: 405 nm, 488 nm (150mW), 568 nm (150mW), 643 nm (150mW)
  • Automated Image Analysis

STEDYCON

  • 30-50nm lateral resol
LEICA STED

gated STED (Stimulated Emission Depletion)

  • Leica TCS SP8 3X
  • 5 color gated STED with ~40-60 nm lateral resolution
  • 3D STED with ~130 nm isotropic resolution
  • Fluorescence channels: 2 x PMT, 2 x HyD Sp GaAsP
  • Laser: tunable WLL 470-670 nm, 405 nm diode laser
  • Depletion laser (STED): 592 nm, 775 nm
  • Fluorescence lifetime upgrade: Falcon & tauSTED
  • Leica Las X & Huygens Deconvolution
LEICA STED

STORM/PALM/STEDYCON

  • Nikon Ti Eclipse with Perfect Focus System
  • Multicolor dSTORM/single particle tracking with 30 nm resolution
  • 3D STORM with 25 nm lateral and 60 nm axial resolution
  • Optosplit II and EMCCD (Andor Ultra 897) for fast multicolor imaging
  • Laser: 405 nm, 488 nm (150mW), 568 nm (150mW), 643 nm (150mW)
  • Automated Image Analysis

STEDYCON

  • 30-50nm lateral resol

EPI-TIRF

  • 4 color Total Internal Reflection Fluorescence microscopy with 100 nm axial Resolution
  • Laser: 405 nm, 488 nm, 568 nm, 647 nm
  • Fast LED excitation with pe4000 (CoolLED)
  • Nikon Ti Eclipse with Perfect Focus System and automatic stage
  • Standard Widefield Imaging with Dapi, GFP, TxRed and Cy5, or phase contrast
  • Prime95B (Photometrics)
  • Heating and CO2 chamber for live-cell imaging
  • Triggered Acquisiti
EPI TIRF

EPI-TIRF

  • 4 color Total Internal Reflection Fluorescence microscopy with 100 nm axial Resolution
  • Laser: 405 nm, 488 nm, 568 nm, 647 nm
  • Fast LED excitation with pe4000 (CoolLED)
  • Nikon Ti Eclipse with Perfect Focus System and automatic stage
  • Standard Widefield Imaging with Dapi, GFP, TxRed and Cy5, or phase contrast
  • Prime95B (Photometrics)
  • Heating and CO2 chamber for live-cell imaging
  • Triggered Acquisiti
    EPI TIRF

Helios 5 CX

  • Focused ion beam scanning electron microscope, FEG, 30kV (2021)
  • Equiped with ETD, ICE, TLD, MD, ICD detectors for BSE, SE and SI imaging
  • Retractable DBS and STEM detectors
  • Carbon and Platinum GIS
  • Gallium FIB

Zeiss 900

  • Transmission electron microscope , 80 kV, (1994)
  • 11 Megapixel Olympus Morada G2 digital camera (2013)

FEI Tecnai G2

  • Transmission electron microscope, FEG, 200 kV (2008)
  • Equipped with Gatan Cryo-holder
  • Tomography holder
  • Gatan CCD-camera (2kx2k)

Helios 5 CX

  • Focused ion beam scanning electron microscope, FEG, 30kV (2021)
  • Equiped with ETD, ICE, TLD, MD, ICD detectors for BSE, SE and SI imaging
  • Retractable DBS and STEM detectors
  • Carbon and Platinum GIS
  • Gallium FIB

Zeiss 900

  • Transmission electron microscope , 80 kV, (1994)
  • 11 Megapixel Olympus Morada G2 digital camera (2013)

FEI Tecnai G2

  • Transmission electron microscope, FEG, 200 kV (2008)
  • Equipped with Gatan Cryo-holder
  • Tomography holder
  • Gatan CCD-camera (2kx2k)

HPM 100

  • High pressure freezer for cryofixation of cellular monolayers, small animals and tissue biopsies

Leica AFS1

  • Automated freeze substitution instrument for low temperature dehydration, postfixation and embedding

FEI Vitrobot

  • Plunge freezing

HPM 100

  • High pressure freezer for cryofixation of cellular monolayers, small animals and tissue biopsies

Leica AFS1

  • Automated freeze substitution instrument for low temperature dehydration, postfixation and embedding

FEI Vitrobot

  • Plunge freezing

Artos 3D

  • Ultramicrotome for serial sectioning

Reichert Ultramicrotome

  • Ultramicrotome for semi and ultrathin room temperature sectioning

UC6 with FC6

  • Ultramicrotome with a cryochamber for ultrathin cryosectioning

CPD 300

  • Critical point dryer

Q150TE

  • Sputter coater for carbon deposition

ACE600

  • Dual ion beam evaporator for high quality carbon and platinum coatings

EMItech K100x

  • Glow discharger
     

Artos 3D

  • Ultramicrotome for serial sectioning

Reichert Ultramicrotome

  • Ultramicrotome for semi and ultrathin room temperature sectioning

UC6 with FC6

  • Ultramicrotome with a cryochamber for ultrathin cryosectioning

CPD 300

  • Critical point dryer

Q150TE

  • Sputter coater for carbon deposition

ACE600

  • Dual ion beam evaporator for high quality carbon and platinum coatings

EMItech K100x

  • Glow discharger
     

Sample Preparations

  • Resin embedding of biological probes for morphological studies
  • RT- and cryo-ultrathin sectioning
  • Pre- and post-embedding immunogold labelling
  • Negative staining
  • TEM and SEM imaging

Special Applications

  • TEM Tomography of resin embedded samples
  • Correlative light and electron microscopy
  • FIB-SEM and Array Tomography

Sample Preparations

  • Resin embedding of biological probes for morphological studies
  • RT- and cryo-ultrathin sectioning
  • Pre- and post-embedding immunogold labelling
  • Negative staining
  • TEM and SEM imaging

Special Applications

  • TEM Tomography of resin embedded samples
  • Correlative light and electron microscopy
  • FIB-SEM and Array Tomography

Flash 1

Workstation

  • Model: Dell Precision T3620
  • OS: Windows 10
  • CPU: 2x 4 Cores - Intel Xeon E3-1270 v5 @ 3.6GHz
  • GPU: 8 GB GDDR5 - Nvidia Quaddro M4000
  • RAM: 64 GB
  • Storage: 1 TB SSD
  • Software: Imaris, Huygens, MATLAB, Microscopy Image Browser (MIB), Fiji, ImageJ, RStudio

Hurricane 2

Workstation

  • Model: Dell Precision T5500
  • Dual Boot OS: Windows 7, Ubuntu 12.04
  • CPU: 2x 6 Cores - Intel Xeon X5660 @ 2.8GHz
  • RAM: 48 GB
  • Storage: 256 GB SSD, 2 TB RAID
  • Software: RapidSTORM 3.2, RapidSTORM 3.3, PyCorr Fit, Fiji, ImageJ, RStudio, Volocity

Imaris

Software

  • Version 8.4.2
  • 3D/4D/5D image visualization and analysis software
  • Powerful 3D rendering
  • Manual and automatic segmentation
  • ImarisVantage analysis and plots
  • Tracking analysis
  • Co-localization analysis
  • ImarisXT interface with MATLAB

Huygens Professional

Software

  • Version 16.10
  • Advanced 3D deconvolution and restoration
  • Supported: widefield, confocal and STED images
  • 3D visualization and analysis
  • Batch processing

Volocity

Software

  • Version 6.0.0
  • Interactive 3D rendering
  • Volumetric measurements
  • Image deconvolution
  • Microscopy control and acquisition
  • FRAP analysis

MATLAB

Software

  • R2017b
  • Software package for automated image and data analysis

Packages available

  • Image Processing and Computer Vision
  • Signal Processing and Communications
  • Math, Statistics and Optimization

Flash 1

Workstation

  • Model: Dell Precision T3620
  • OS: Windows 10
  • CPU: 2x 4 Cores - Intel Xeon E3-1270 v5 @ 3.6GHz
  • GPU: 8 GB GDDR5 - Nvidia Quaddro M4000
  • RAM: 64 GB
  • Storage: 1 TB SSD
  • Software: Imaris, Huygens, MATLAB, Microscopy Image Browser (MIB), Fiji, ImageJ, RStudio

Hurricane 2

Workstation

  • Model: Dell Precision T5500
  • Dual Boot OS: Windows 7, Ubuntu 12.04
  • CPU: 2x 6 Cores - Intel Xeon X5660 @ 2.8GHz
  • RAM: 48 GB
  • Storage: 256 GB SSD, 2 TB RAID
  • Software: RapidSTORM 3.2, RapidSTORM 3.3, PyCorr Fit, Fiji, ImageJ, RStudio, Volocity

Imaris

Software

  • Version 8.4.2
  • 3D/4D/5D image visualization and analysis software
  • Powerful 3D rendering
  • Manual and automatic segmentation
  • ImarisVantage analysis and plots
  • Tracking analysis
  • Co-localization analysis
  • ImarisXT interface with MATLAB

Huygens Professional

Software

  • Version 16.10
  • Advanced 3D deconvolution and restoration
  • Supported: widefield, confocal and STED images
  • 3D visualization and analysis
  • Batch processing

Volocity

Software

  • Version 6.0.0
  • Interactive 3D rendering
  • Volumetric measurements
  • Image deconvolution
  • Microscopy control and acquisition
  • FRAP analysis

MATLAB

Software

  • R2017b
  • Software package for automated image and data analysis

Packages available

  • Image Processing and Computer Vision
  • Signal Processing and Communications
  • Math, Statistics and Optimization

Research

The central aim of our group is to establish suitable single cell techniques and the application of the most diverse microscopic methods to study the following processes: (i) Molecular composition, nanoscale organization and physiological function of tight junction as ionselective paracellular barrier (with Volker Haucke) (ii) Establish an integrated model of mitochondria based on cross-linking MS, Super-resolution and electron microscopy (with Fan Liu) (iii) Identification of toxic compounds and new pharmacological mechanisms via high-resolution confocal imaging (with Han Sun & Jens Peter von Kries).

Our team

Publications via ORCID

of 5

Aurora Kinase A Is Involved in Controlling the Localization of Aquaporin-2 in Renal Principal Cells

  • Sandrine Baltzer; Timur Bulatov; Christopher Schmied; Andreas Krämer; Benedict-Tilman Berger; Andreas Oder; Ryan Walker-Gray; Christin Kuschke; Kerstin Zühlke; Jenny Eichhorst; Martin Lehmann; Stefan Knapp; John Weston; Jens Von Kries; Roderich D. Süssmuth; Enno Klussmann

International Journal of Molecular Sciences 2022

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Selective endocytosis of Ca 2+ -permeable AMPARs by the Alzheimer’s disease risk factor CALM bidirectionally controls synaptic plasticity

  • Domenico Azarnia Tehran; Gaga Kochlamazashvili; Niccolò P. Pampaloni; Silvia Sposini; Jasmeet Kaur Shergill; Martin Lehmann; Natalya Pashkova; Claudia Schmidt; Delia Löwe; Hanna Napieczynska; Arnd Heuser; Andrew J. R. Plested; David Perrais; Robert C. Piper; Volker Haucke; Tanja Maritzen

Science Advances 2022

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The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane

  • Claudia Matthaeus; Kem A. Sochacki; Andrea M. Dickey; Dmytro Puchkov; Volker Haucke; Martin Lehmann; Justin W. Taraska

Nature Communications 2022

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Nanoscale segregation of channel and barrier claudins enables paracellular ion flux

  • Hannes Gonschior; Christopher Schmied; Rozemarijn Eva van der Veen; Jenny Eichhorst; Nina Himmerkus; Jörg Piontek; Dorothee Günzel; Markus Bleich; Mikio Furuse; Volker Haucke; Martin Lehmann

Nature Communications 2022

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Proton-gated anion transport governs macropinosome shrinkage

  • Mariia Zeziulia; Sandy Blin; Franziska W. Schmitt; Martin Lehmann; Thomas J. Jentsch

Nature Cell Biology 2022

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N-Methyl deuterated rhodamines for protein labelling in sensitive fluorescence microscopy

  • Kilian Roßmann; Kerem C. Akkaya; Pascal Poc; Corentin Charbonnier; Jenny Eichhorst; Hannes Gonschior; Abha Valavalkar; Nicolas Wendler; Thorben Cordes; Benjamin Dietzek-Ivansic; Benjamin Jones; Martin Lehmann; Johannes Broichhagen

Chemical Science 2022

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Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy

  • Michael Trumpp; Anna Oliveras; Hannes Gonschior; Julia Ast; David Hodson; Petra Knaus; Martin Lehmann; Melissa Birol; Johannes Broichhagen

Chemical Communications 2022

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Mutant Phosphodiesterase 3A Protects From Hypertension-Induced Cardiac Damage.

  • Ercu M; Michael Benedikt Mücke; Pallien T; Lajos Markó; Anastasiia Sholokh; Schächterle C; Aydin A; Kidd A; Stephan Walter; Esmati Y; Brandon McMurray; Daniella F Lato; Daniele Yumi Sunaga-Franze; Philip Dierks; Flores BIM; Walker-Gray R; Maolian Gong; Merticariu C; Zühlke K; Russwurm M; Liu T; Theda Ulrike Patricia Bartolomaeus; Pautz S; Schelenz S; Taube M; Napieczynska H; Heuser A; Eichhorst J; Lehmann M; Duncan C. Miller; Diecke S; Qadri F; Popova E; Langanki R; Movsesian MA; Friedrich W. Herberg; Forslund SK; Dominik N Muller; Borodina T; Maass PG; Sylvia Bähring; Hübner N; Bader M; Enno Klussmann

Circulation 2022

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Expanded LUXendin Color Palette for GLP1R Detection and Visualization In Vitro and In Vivo

  • Julia Ast; Alissa N. Novak; Tom Podewin; NHF Fine; Benjamin Jones; Alejandra Tomas; Ramona Birke; Kilian Roßmann; Bettina Mathes; Jenny Eichhorst; Martin Lehmann; Amelia Linnemann; David Hodson; Johannes Broichhagen

JACS Au 2022

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Sulfonated red and far-red rhodamines to visualize SNAP- and Halo-tagged cell surface proteins

  • Martin Lehmann

Organic & Biomolecular Chemistry 2022

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of 10

Photocaged Hoechst Enables Subnuclear Visualization and Cell Selective Staining of DNA in vivo

  • Carina A. Lämmle; Adam Varady; Thorsten G. Müller; Caterina Sturtzel; Michael Riepl; Bettina Mathes; Jenny Eichhorst; Anje Sporbert; Martin Lehmann; Hans‐Georg Kräusslich; Martin Distel; Johannes Broichhagen

ChemBioChem 2021

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Rab2 regulates presynaptic precursor vesicle biogenesis at the trans-Golgi

  • Torsten W.B. Götz; Dmytro Puchkov; Veronika Lysiuk; Janine Lützkendorf; Alexander G. Nikonenko; Christine Quentin; Martin Lehmann; Stephan J. Sigrist; Astrid G. Petzoldt

Journal of Cell Biology 2021

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Zellpermeable Nanobodys ermöglichen Zwei‐Farben‐Superauflösungsmikroskopie in lebenden, nicht transfizierten Zellen

  • Anselm F. L. Schneider; Laila S. Benz; Martin Lehmann; Christian P. R. Hackenberger

Angewandte Chemie 2021

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Cell‐Permeable Nanobodies Allow Dual‐Color Super‐Resolution Microscopy in Untransfected Living Cells

  • Anselm F. L. Schneider; Laila S. Benz; Martin Lehmann; Christian P. R. Hackenberger

Angewandte Chemie International Edition 2021

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Cellular uptake of large biomolecules enabled by cell-surface-reactive cell-penetrating peptide additives

  • Anselm F. L. Schneider; Marina Kithil; M. Cristina Cardoso; Martin Lehmann; Christian P. R. Hackenberger

Nature Chemistry 2021

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Identification of disease-relevant modulators of the SHH pathway in the developing brain.

  • Mecklenburg N; Izabela Kowalczyk; Witte F; Görne J; Laier A; Mamo TM; Hannes Gonschior; Lehmann M; Richter M; Sporbert A; Purfürst B; Hübner N; Annette Hammes

Development (Cambridge, England) 2021

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of 6

The Unc13A isoform is important for phasic release and olfactory memory formation at mushroom body synapses.

  • Woitkuhn J; Ender A; Beuschel; Maglione M; Matkovic-Rachid T; Huang S; Lehmann M; Geiger JRP; Sigrist SJ

Journal of neurogenetics 2020

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Interrogating surface <i>versus</i> intracellular transmembrane receptor populations using cell-impermeable SNAP-tag substrates.

  • Pascal Poc; Gutzeit VA; Julia Ast; Joon Lee; Benjamin Jones; Elisa D'Este; Mathes B; Lehmann M; David Hodson; Levitz J; Johannes Broichhagen

Chemical science 2020

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Phosphodiesterase 3A and Arterial Hypertension.

  • Ercu M; Lajos Markó; Schächterle C; Tsvetkov D; Cui Y; Maghsodi S; Theda Ulrike Patricia Bartolomaeus; Maass PG; Zühlke K; Gregersen N; Hübner N; Hodge R; Mühl A; Pohl B; Illas RM; Geelhaar A; Walter S; Napieczynska H; Schelenz S; Taube M; Heuser A; Anistan YM; Qadri F; Mihail Todiras; Plehm R; Popova E; Langanki R; Eichhorst J; Lehmann M; Wiesner B; Russwurm M; Sofia K Forslund; Kamer I; Müller DN; Gollasch M; Aydin A; Bähring S; Michael Bader; Luft FC; Klussmann E

Circulation 2020

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EHD2-mediated restriction of caveolar dynamics regulates cellular fatty acid uptake

  • Claudia Matthaeus; Ines Lahmann; Severine Kunz; Wenke Jonas; Arthur Alves Melo; Martin Lehmann; Elin Larsson; Richard Lundmark; Matthias Kern; Matthias Blüher; Hannah Olschowski; Julian Kompa; Britta Brügger; Dominik N. Müller; Volker Haucke; Annette Schurmann; Carmen Birchmeier; Oliver Daumke

Proceedings of the National Academy of Sciences 2020

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Super-Resolution Imaging of Tight and Adherens Junctions: Challenges and Open Questions

  • Hannes Gonschior; Volker Haucke; Martin Lehmann

International Journal of Molecular Sciences 2020

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of 5

RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses.

  • Marisa M Brockmann; Maglione M; Willmes CG; Alexander Stumpf; Bouazza BA; Velasquez LM; M. Katharina Grauel; Beed P; Lehmann M; Niclas Gimber; Schmoranzer J; Stephan Sigrist; Christian Rosenmund; Dietmar Schmitz

eLife 2019

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eNOS-NO-induced small blood vessel relaxation requires EHD2-dependent caveolae stabilization

  • Claudia Matthäus; Xiaoming Lian; Séverine Kunz; Martin Lehmann; Cheng Zhong; Carola Bernert; Ines Lahmann; Dominik N. Müller; Maik Gollasch; Oliver Daumke

PLOS ONE 2019

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Rapid active zone remodeling consolidates presynaptic potentiation.

  • Mathias Böhme; Anthony William McCarthy; Andreas Grasskamp; Beuschel; Goel P; Jusyte M; Laber D; Huang S; Rey U; Petzoldt AG; Lehmann M; Göttfert F; Haghighi P; Hell SW; David Owald; Dickman D; Sigrist SJ; Alexander Matthias Walter

Nature communications 2019

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Homeostatic scaling of active zone scaffolds maintains global synaptic strength

  • Pragya Goel; Dominique Dufour Bergeron; Mathias A. Böhme; Luke Nunnelly; Martin Lehmann; Christopher Buser; Alexander Matthias Walter; Stephan Sigrist; Dion Dickman

Journal of Cell Biology 2019

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Nanoscale coupling of endocytic pit growth and stability

  • Martin Lehmann; Ilya Lukonin; Frank Noé; Jan Schmoranzer; Cecilia Clementi; Dinah Loerke; Volker Haucke

Science Advances 2019

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A SEPT1-based scaffold is required for Golgi integrity and function.

  • Kyungyeun Song; Claudia Gras; Capin G; Niclas Gimber; Lehmann M; Mohd S; Dmytro Puchkov; Rödiger M; Wilhelmi I; Daumke O; Schmoranzer J; Annette Schurmann; Michael Krauss

Journal of cell science 2019

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Phosphorylation of the Bruchpilot N-terminus unlocks axonal transport of active zone building blocks

  • Jan H. Driller; Janine Lützkendorf; Harald Depner; Matthias Siebert; Benno Kuropka; Christoph Weise; Chengji Piao; Astrid G. Petzoldt; Martin Lehmann; Ulrich Stelzl; René Zahedi; Albert Sickmann; Christian Freund; Stephan Sigrist; Markus Wahl

Journal of Cell Science 2019

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Autophagy within the mushroom body protects from synapse aging in a non-cell autonomous manner

  • Anuradha Bhukel; Beuschel; Marta Maglione; Martin Lehmann; Gabor Juhász; Frank Madeo; Stephan J. Sigrist

Nature Communications 2019

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