Cellular Imaging

Equipment

Light Microscopy

Confocal Laser Scanning Microscopy (CLSM)

LSM510-META-UV  
  • Fluorescence channels: 3 - one is a META detector
  • Laser: 364 nm, 458 nm, 488 nm, 514 nm, 543 nm, 633 nm
  • Heating table unit

LSM510-META-NLO  
  • Fluorescence channels: 3 - one is a META detector
  • Laser: 458 nm, 488 nm, 514 nm, 543 nm, 633 nm and a tunable IR-laser
  • Multiphoton excitation: 720 - 930 nm
  • Equipped with a FLIM-setup (Becker & Hickl GmbH)
  • Heating table unit

LSM510-ConfoCor2  
  • Fluorescence channels: 3
  • Laser: 458 nm, 488 nm, 514 nm, 543 nm, 633 nm
  • Equipped with a FCS-setup (Fluorescence Correlation Spectroscopy)
  • Heating table unit

LSM710-ConfoCor3  
  • Fluorescence channels: 34-Channel QUASAR Detection Unit
  • Laser: 405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 633 nm
  • Equipped with a FCS-setup (Fluorescence Correlation Spectroscopy)
  • Definite Focus
  • Heating table unit

LSM780  
  • Fluorescence channels: 34-Channel QUASAR Detection Unit
  • Laser: 405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 594 nm, 633 nm
  • Definite Focus
  • Heating table unit

Spinning Disc Systems

ZEISS-CSU  
  • Perkin Elmer UltraView
  • Yokogawa Spinning disc
  • EMCCD Camera
  • Laser: 405 nm, 488 nm, 568 nm, 640 nm
  • Piezo Z-drive for fast 3D Imaging
  • FRAP unit
  • Velocity Software and analysis

Nikon-CSU  
  • Nikon Ti Eclipse with Perfect Focus System and automatic stage
  • Yokogawa Spinning disc (CSU-X1)
  • EMCCD Camera (Andor AU-888)
  • Laser: 405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 638 nm
  • Fast triggered aquisition
  • Piezo Z-drive for fast 3D imaging
  • FRAPPA and 16-Color LED for FRAP and fast optogenetics
  • Incubator with temperature and CO2 control (OKOLAB)
  • NIS-Elements for control and analysis

Super-Resolution Microscopy

gSTED  
  • Leica SP8 with LAS X software
  • 3 color gateSTED with ~50 nm lateral resolution
  • Fluorescence channels: 4 (2 x PMT, 2 x HyD Sp GaAsP)
  • Laser: tunable WWL 470-670 nm, 405 nm diode laser
  • Depletion laser (STED): 592 nm, 775 nm
  • Huygens Deconvolution

STORM/PALM  
  • Nikon Ti Eclipse with Perfect Focus System
  • Multicolor dSTORM with 30 nm resolution
  • 3D STORM with 25 nm lateral and 60 nm axial resolution
  • Optosplit II and EMCCD (Andor Ultra 897) for fast multicolor imaging
  • Laser: 405 nm, 488 nm (150mW), 568 nm (150mW), 643 nm (150mW)
  • Automated Image Analysis

Widefield and TIRF Microscopy

EPI-TIRF  
  • 4 color Total Internal Reflection Fluorescence microscopy with 100 nm axial Resolution
  • Standard Widefield Imaging with Dapi, GFP, TxRed and Cy5 Filter sets
  • Nikon Ti Eclipse with Perfect Focus System and an automatic stage
  • sCMOS Camera (Andor mNeo)
  • Laser: 405 nm, 488 nm, 568 nm, 647 nm
  • Heating and CO2 chamber for live-cell imaging
  • Detailed manual available

EPI-pHlourin  
  • Sensitive Widefield Imaging with Dapi, GFP, TxRed and Cy5 Filter sets
  • Nikon Ti Eclipse with Perfect Focus System and an automatic stage
  • sCMOS Camera (Andor mNeo)
  • Heating and CO2 chamber for live-cell imaging
  • Perfusion system
  • Detailed manual available

EPI-pHast  
  • Very sensitive Widefield Imaging with Dapi, GFP and Cy5 Filter sets
  • Zeiss Observer with Definite Focus System
  • Sensitive EMCCD Camera (Hamamatsu)
  • Heating chamber for live-cell imaging
  • Micromanager Control

EPI-FIX & FOXY  
  • Simple Widefield Imaging with Dapi, GFP, Cy3 and Cy5 Filter sets
  • Zeiss Axiovert 200M
  • CCD Camera (Zeiss)
  • Axiovision or Micromanager Control

Flow Cytometry

BD LSR Fortessa  
  • Fluorescence channles: 21
  • Laser: 405 nm, 445 nm, 488 nm, 561 nm, 640 nm
  • High throughput sampler (96-well plates)

Bioimage Analysis

Flash 1

Workstation

 
  • Model: Dell Precision T3620
  • OS: Windows 10
  • CPU: 2x 4 Cores - Intel Xeon E3-1270 v5 @3.6GHz
  • GPU: 8 GB GDDR5 - Nvidia Quaddro M4000
  • RAM: 64 GB
  • Storage: 1 TB SSD
  • Software: Imaris, Huygens, MATLAB, Microscopy Image Browser (MIB), Fiji, ImageJ, RStudio

Hurricane 2

Workstation

 
  • Model: Dell Precision T5500
  • Dual Boot OS: Windows 7, Ubuntu 12.04
  • CPU: 2x 6 Cores - Intel Xeon X5660 @ 2.8GHz
  • RAM: 48 GB
  • Storage: 256 GB SSD, 2 TB RAID
  • Software: RapidSTORM 3.2, RapidSTORM 3.3, PyCorr Fit, Fiji, ImageJ, RStudio, Volocity

Imaris

8.4.2

 
  • 3/4D Image Visulization and Analysis software
  • Powerful 3D rendering
  • Manual and automatic segmentation
  • ImarisVantage analysis and plots
  • Tracking analysis
  • Co-localization analysis
  • ImarisXT interface with MATLAB

Volocity

6.0.0

 
  • Visualization - interactive 3D rendering
  • Quantitation - volumetric measurements
  • Restoration - image deconvolution
  • Acquisition - microscopy control and acquisition
  • FRAP analysis

Huygens

16.10

 
  • Huygens Professional
  • Advanced 3D Deconvolution
  • Broad set of image restoration algorithms
  • For widefield, confocal and STED iamges
  • 3D visualization and analysis
  • Batch Processing

MATLAB

R2017b

 
  • Software package for automated Image and Data analysis
  • Image Processing and Computer Vision
  • Signal Processing and Communications
  • Math, Statistics and Optimization

Electron Microscopy

Zeiss 900  
  • Transmission electron microscope , 80 kV, (1994)
  • 11 Megapixel Olympus Morada G2 digital camera (2013)

FEI Tecnai G2  
  • Transmission electron microscope, FEG, 200 kV (2008)
  • Equipped with Gatan Cryo-holder
  • Tomography holder
  • Gatan CCD-camera (2kx2k)

Sample preparation  
  • Resin embedding of biological probes for morphological studies
  • Room temperature or freeze substitution techniques
  • Tokuyasu cryo-embedding and cryo-sectioning technique
  • RT- and cryo-sectioning
  • Pre- and post-embedding immunogold labelling
  • Negative staining of protein particles, two-dimensional protein crystals or fibril structures (standard and sandwich techniques)
  • Preparation of formvar and carbon coated EM grids
  • Vitrobot (FEI, 2010) for vitrification
  • Glow discharge and carbon coating device
  • RT-ultramicrotome and Cryo-ultramicrotome

Special techniques  
  • Tomography of resin embedded samples
  • Imaging of vitrified (fast frozen, embedded in amorphous ice) samples at -170°C using low dose techniques

Leibniz-Forschungsinstitut für Molekulare Pharmakologie im Forschungsverbund Berlin e.V. (FMP)
Campus Berlin-Buch
Robert-Roessle-Str. 10
13125 Berlin, Germany
+4930 94793 - 100 
+4930 94793 - 109 (Fax)
info(at)fmp-berlin.de

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