Prof. Dr. Christian Hackenberger

phone +49 30 94793 181

Peptide Chemistry

The core facility was formed in February 2013 as a service unit for peptide synthesis and is part of the department of Chemical Biology II. Christian Hackenberger contributes his expertise for the synthesis of advanced post-translationally modified polypeptides as well as unnatural peptide -conjugates. Rudolf Volkmer brings in his experience in peptide synthesis, peptide libraries and peptide arrays on solid supports. Ines Kretzschmar takes care for the execution of the peptide synthesis procedure.

Our aim is to provide synthetic peptides to all research groups at the FMP and users of this facility pay a fee for this service, but overall cost is low. The facility is equipped with two parallel peptide synthesizers and a microwave-assisted synthesizer for challenging peptide sequences, one analytical and one preparative RP-HPLC instruments as well as a MALDI-ToF mass spectrometer.

The full repertoire of standard SPPS methods is employed in this facility to generate linear peptides with or without side chain and / or termini-modifications. More challenging peptides are also made including dye-labeled peptides, head-to-tail or side chain-to-side chain cyclic peptides with and without dye-labeling. Furthermore, peptides containing unnatural amino acids, building blocks or proline-mimetics, D-amino acids, polyethylene glycol chains and phosphorylated amino acids are synthesized. Up to now this facility synthesized 1537 peptides, an average of 192 peptides/year.

In addition to its services, this core facility is embedded in several FMP research projects. For example, our cellulose membrane bound peptide arrays are useful tools to investigate how TARPs modulate AMPA receptor gating, which is investigated in details by the Plested lab.  Furthermore, this facility is involved in the project of Pro-Pro dipeptide mimics in context of the VIP BMBF project ProMiCom, coordinated by Ronald Kühne. Additionally, we optimize the synthesis of cyclic cell-penetrating peptides (cCPPs), which have been applied to the delivery of functional nanobodies into specific intracellular compartments by the Hackenberger lab.

Selected publications  

1. Riva I., Eibl C.,Volkmer R., Carbone A.L., Plested A.J. Control of AMPA receptor activity by the extracellular loops of auxiliary proteins. Elife 6, e28680 (2017)

2.  Barone M, Müller M, Chiha S, Ren J, Albat D, Soicke A, Dohmen S, Klein M, Bruns J, van Dinther M, Opitz R, Lindemann P, Beerbaum M, Motzny K, Roske Y, Schmieder P, Volkmer R, Nazaré M, Heinemann U, Oschkinat H, Ten Dijke P, Schmalz HG, Kühne R. Designed nanomolar small-molecule inhibitors of Ena/VASP EVH1 interaction impair invasion and extravasation of breast cancer cells. Proc Natl Acad Sci USA 117, 29684 (2020)

3. Herce H.D., Schumacher D., Schneider A.F.L., Ludwig A.K., Mann F.A., Fillies M., Kasper M.-A., Reinke S., Krause E., Leonhardt H., Cardoso M.C., Hackenberger C.P.R. Cell-permeable nanobodies for targeted immunolabelling and antigen manipulation in living cells. Nature Chemistry 9, 762 (2017)

4. Penkert M., Hauser A., Harmel R., Fiedler D., Hackenberger C.P.R., KrauseE. Electron Transfer/Higher Energy Collisional Dissociation of Doubly Charged Peptide Ions: Identification of Labile Protein Phosphorylations. J Am Soc Mass Spectrom 30, 1578 (2019)

5. Mertens M., Hilsch M., Haralampiev I., Volkmer R., Wessig P., Müller P. Synthesis and characterization of a new bifunctionalized, fluorescent, and amphiphilic molecule for recruiting SH-containing molecules to membranes. Chembiochem 19, 1643 (2018)

Leibniz-Forschungsinstitut für Molekulare Pharmakologie im Forschungsverbund Berlin e.V. (FMP)
Campus Berlin-Buch
Robert-Roessle-Str. 10
13125 Berlin, Germany
+4930 94793 - 100 
+4930 94793 - 109 (Fax)

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