Department Chemical Biology I (Dorothea Fiedler)

Group Publication


Mao Li, Robert Puschmann, Andreas Herdlitschka, Dorothea Fiedler, Helma Wennemers. Delivery of myo‐Inositol Hexakisphosphate to the Cell Nucleus with a Proline‐Based Cell‐Penetrating Peptide. Angewandte 2020, 59:15586-15589

David Furkert, Sarah Hostachy, Michal Nadler-Holly, Dorothea Fiedler. Triplexed Affinity Reagents to Sample the Mammalian Inositol Pyrophosphate Interactome. Cell Chem. Bio 2020, 27: 1-12

Soumyadip Sahu, Zhenzhen Wang, Xinfu Jiao, Chunfang Gu, Nikolaus Jork, Christopher Wittwer, Xingyao Li, Sarah Hostachy, Dorothea Fiedler, Huanchen Wang, Henning J. Jessen, Megerditch Kiledjian, Stephen B. Shears. InsP7 is a small-molecule regulator of NUDT3-mediated mRNA decapping and processing-body dynamics.PNAS2020, 117 (32):19245–19253.

Xingyao Li, Chunfang Gu, Sarah Hostachy, Soumyadip Sahu, Christopher Wittwer, Henning J. Jessen, Dorothea Fiedler, Huanchen Wang, Stephen B. Shears. Control of XPR1-dependent cellular phosphate efflux by InsP8 is an exemplar for functionally-exclusive inositol pyrophosphate signaling. PNAS2020, 117 (7):3568-3574

Robert Puschmann, Robert K. Harmel, Dorothea Fiedler. Analysis of metabolically labeled inositol phosphate messengers by NMR. Methods Enzymol. 2020, 641:35-52


Robert Puschmann, Robert K. Harmel and Dorothea Fiedler. Scalable Chemoenzymatic Synthesis of Inositol Pyrophosphates. Biochemistry2019, 58(38):3927-3932  ACS Editors' Choice

Jinsheng Zhu, Kelvin Lau, Robert Puschmann, Robert K Harmel, Youjun Zhang, Verena Pries, Philipp Gaugler, Larissa Broger, Amit K Dutta, Henning J Jessen, Gabriel Schaaf, Alisdair R Fernie, Ludwig A Hothorn, Dorothea Fiedler, Michael Hothorn. Two bifunctional inositol pyrophosphate kinases/phosphatases control plant phosphate homeostasis. eLife2019, 8:e43582

Martin Penkert, Anett Hauser, Robert Harmel, Dorothea Fiedler, Christian P. R. Hackenberger, Eberhard Krause. "Electron Transfer/Higher Energy Collisional Dissociation of Doubly Charged Peptide Ions: Identification of Labile Protein Phosphorylations" J. Am. Soc. Mass Spectrom.2019 30(9):1578-1585

Robert K. Harmel, Robert Puschmann, Minh Nguyen Trung, Adolfo Saiardi, Peter Schmieder, Dorothea Fiedler. "Harnessing 13C-labeled myo-inositol to interrogate inositol phosphate messengers by NMR" Chem. Sci.201910(20):5267-5274


Alan M. Marmelstein, Jeremy A. M. Morgan, Martin Penkert, Daniel T. Rogerson, Eberhard Krause, Jason W Chin and Dorothea Fiedler. "Pyrophosphorylation via Selective Phosphoprotein Derivatization." Chem. Sci. 2018, 9, 5929-3596.

Cole M. Dovey, Jonathan Diep, Bradley P. Clarke, Andrew T. Hale, Dan E. McNamara, Hongyan Guo, Nathaniel W. Brown Jr., Jennifer Yinuo Cao, Christy R. Grace, Peter J. Gough, John Bertin, Scott J. Dixon, Dorothea Fiedler, Edward S. Mocarski, William J. Kaiser, Tudor Moldoveanu, John D. York, Jan E. Carette. "MLKL Requires the Inositol Phosphate Code to Execute Necroptosis." Mol Cell. 2018, 70, 936-948.

Robert Harmel & Dorothea Fiedler. "Features and regulation of non-enzymatic post-translational modifications" Nature Chemical Biology2018, 14, 244-252


Martin Penkert, Lisa M. Yates, Michael Schümann, David Perlman, Dorothea Fiedler, Eberhard Krause. "Unambiguous Identification of Serine and Threonine Pyrophosphorylation Using Neutral-Loss-Triggered Electron-Transfer/Higher-Energy Collision Dissociation." Analytical Chemistry2017, 89(6), 3672-3680

Alan M Marmelstein, Javier Moreno, Dorothea Fiedler. "Chemical Approaches to Studying Labile Amino Acid Phosphorylation." Top Curr Chem (J)2017, 375(2):22


Mingxuan Wu, Lucy S. Chong, David H. Perlman, Adam C. Resnick, Dorothea Fiedler. "Inositol Polyphosphates intersect with signalling and metabolic networks via two distinct mechanisms." PNAS2016, 113(44), E6757-6765

Manasa Chanduri, Ashim Rai, Aushaq Bashir Malla, Mingxuan Wu, Dorothea Fiedler, Roop Mallik, Rashna Bhandari. "Inositol hexakisphosphate kinase 1 (IP6K1) activity is required for cytoplasmic dynein-driven transport." Biochemical Journal2016, 473(19) 3031-304

Anastasia Hager, Mingxuan Wu, Huanchen Wang, Nathaniel W. Brown Jr., Stephen B Shears, NicolásVeiga, Dorothea Fiedler. "Cellular Cations Control Conformational Switching of Inositol Pyrophosphate Analogues." Chem. Eur. J.2016, 22, 12406-12414 (Hot Paper)

Nathaniel W. Brown, Alan M. Marmelstein, Dorothea Fiedler. "Chemical tools for interrogating inositol pyrophosphate structure and function." Chem. Soc. Rev.2016, 45, 6311-26

Lisa M. Yates and Dorothea Fiedler. "A Stable Pyrophosphoserin Analog for Incorporation into Peptides and Proteins." ACS Chem Biol.2016, 11(4), 1066-1073

Elizabeth A. Steidle, Lucy S. Chong, Mingxuan Wu, Elliot Crooke, Dorothea Fielder, Adam C. Resnick, Ronda J. Rolfes. "A novel inostiol pyrophosphate phosphatase in Saccharomyces cerevisiae: Siw14 selectively cleaves the β-phosphate from 2-diphosphoinositol pentakisphosphate (5PP-IP5)" J. Biol. Chem.2016, 291(13), 6772-83


Florence J. Williams and Dorothea Fiedler. "A Fluorescent Sensor and Gel Stain for Detection of Pyrophosphorylated Proteins" ACS Chem. Biol.2015, 10(9), pp 1958-1963

John H. Conway and Dorothea Fiedler. "An Affinity Reagent for the Recognition of Pyrophosphorylated Peptides" Angew. Chem. Int. Ed.2015, 54, 3941-3945.

Lisa M. Yates and Dorothea Fiedler. "Establishing the Stability and Reversability of Protein Pyrophosphorylation with Synthetic Peptides" ChemBioChem2015, 16, 415-423. (Cover Picture, Highlighted in ChemViews Magazine: Lost in Post-Translation)


Lisa M. Yates, Alan M. Marmelstein, and Dorothea Fiedler. "Synthetic Pyrophosphorylation Methods and Their Use in Chemical Biology." Synlett2014, 25, 2239-2245.

Mingxuan Wu, Lucy S. Chong, Samanta Capolicchio, Henning J. Jessen, Adam C. Resnick, and Dorothea Fiedler. "Elucidation Diphosphoinositol Polyphosphate Funktion with Nonhydrolyzable Analogues" Angew. Chem. Int. Ed. 2014, 53, 7192-7197.

Feng Roa, Jiyoung Cha, Jing Xu, Risheng Xu, M. Scott Vandiver, Richa Tyagi, Robert Tokhunts, Michael A. Koldobskiy, Chenglai Fu, Roxanne Barrow, Mingxuan Wu, Dorothea Fiedler, James C. Barrow, Solomon H. Snyder. "Inositol Pyrophosphates Mediate the DNA-PK/ATM-p53 Cell Death Pathway by Regulating CK2 Phosphorylation of Tti1/Tti2." Mol. Cell2014, 54, 119-132.

Alan M. Marmelstein, Lisa M. Yates, John H. Conway, and Dorothea Fiedler. "Chemical Pyrophosphorylation of Functionally Diverse Peptides." J. Am. Chem. Soc. 2014, 136, 108-111. (Highlighted in JACS Spotlights: J. Am. Chem. Soc. 2014, 136, 108-111.)


Minxuan Wu, Barbara E. Dul, Alexandra J. Trevisan, and Dorothea Fiedler. "Synthesis and characterization of non-hydrolysable diphosphoinositol polyphosphate messengers." Chem. Sci.2013, 4, 405-410.

Previous Publications


J. I. Kliegman, D. Fiedler, C. J. Ryan, Y. F. Xu, X. Y. Su, D. Thomas, M. C. Caccese, A. Cheng, M. Shales, J. D. Rabinowitz, N. J. Krogan, K. M. Shokat. " Chemical Genetics of Rapamycin-Insensitive TORC2 in S. cerevisiae." Cell Reports 2013, 5, 1725–1736.

J. L. Nakamura, C. Phong, E. Pinarbasi, S. C. Kogan, S. Vandenberg, A. E. Horvai, B. A. Faddegon, D. Fiedler, K. M. Shokat, B. T. Houseman, R. Chao, R. O. Pieper, K. Shannon. “Dose-dependent effects of focal fractionated irradiation on secondary malignant neoplasms in Nf1 mutant mice.” Cancer Res.2011, 71, 106-15.

S. van Wageningen, P. Kemmeren, P. Lijnzaad, T. Margaritis, J. J. Benschop, I. J. de Castro, D. van Leenen, M. J. A. Groot Koerkamp, C. W. Ko, A. J. Miles, N. Brabers, M.   O. Brok, T. L. Lenstra, D. Fiedler, L. Fokkens, R. Aldecoa, E. Apweiler, V. Taliadouros, K. Sameith, L. A. L. van de Pasch, S. R. van Hooff, L. V. Bakker, N. J. Krogan, B. Snel, F.   C. P. Holstege. “Functional overlap and regulatory links shape genetic interactions between signaling pathways.” Cell2010, 143, 991-1004. Highlighted in Cell2010, 143, 867-869. 

S. Bandyopadhyay, M. Mehta, D. Kuo, M. K. Sung, R. Chuang, E. J. Jaehnig, B> Bodenmiller, K. Licon, W. Copeland, M. Shales, D. Fiedler, J. Dutkowski, A. Guénolé, H. van Attikum, K. M. Shokat, R. D. Kolodner, W. K. Huh, R. Aebersold, M. C. Keogh, N. J. Krogan, T. Ideker. “Rewiring of genetic networks in response to DNA damage.” Science2010, 330, 1385-1389.

P. Beltrao, J. C. Trinidad, D. Fiedler, A. Roguev, W. A. Lim, K. M. Shokat, A. L. Burlingame, N. J. Krogan. “Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.” PLoS Biol.2009, 7, e1000134.

D. Fiedler , H. Braberg, M. Mehta, G. Chechik, G. Cagney, P. Mukherjee, A. C. Silva, M. Shales, S. R. Collins S. van Wageningen, P. Kemmeren, F. C. P. Holstege, J. S. Weissman, M. Christopher-Keogh, D. Koller, K. M. Shokat, N. J. Krogan. “Functional organization of the S. cerevisiae phosphorylation network.” Cell 2009136, 952- 963.  Cover Picture

T. Okuzumi, D. Fiedler, C. Zhang, D. C. Gray, B. Aizenstein, R. Hoffman, K. M. Shokat. “Inhibitor hijacking of Akt activation.” Nat. Chem. Bio. 20095, 484-493.

H. Wang, B. Kakaradov, S. R. Collins, L. Karotki, D. Fiedler, M. Shales, K. M. Shokat, T. Walther, N. J. Krogan, D. Koller. “A complex-based reconstruction of the S. cerevisiae interactome.“ Mol. Cell. Proteomics 20098, 1361-1381.

L. M. Sly; M. J. Hamilton; E. Kuroda; V. W. Ho; F. L. Antignano; S. L. Omeis; C. J. van Netten-Thomas; D. Wong; H. K. Brugger; O. Williams; M. E. Feldman; B. T. Houseman; D. Fiedler; K. M. Shokat; G. Krystal. “ SHIP prevents lipopolysaccharide from triggering an antiviral response in mice.” Blood 2009113, 2945-2954.

J. Wang, Z. Knight, D. Fiedler, O. R. Williams, K. M. Shokat, D. Pearce. “Activity of the p110-alpha subunit of phosphatidylinositol-3-kinase is required for activation of epithelial sodium transport.” Am. J. Physiol. 2008295, F843-F850. 

T. Tamguney, C. Zhang, D. Fiedler, K. M. Shokat, D. Stokoe. “Analysis of 3-phosphoinositide-dependent kinase-1 signaling and function in ES Cells.” Exp. Cell Res. 2008314, 2299-2312.


J. S. Mugridge, D. Fiedler, K. N. Raymond. “A Ferrocene-Based Catecholamide Ligand: the Consequences of Ligand Swivel for Directed Supramolecular Self-Assembly.” J. Coord. Chem.2010, 63, 2779 – 2789.

M. Pluth, D. Fiedler, R. G. Bergman, K. N. Raymond. “Molecular recognition and self-assembly special feature: Encapsulation and characterization of proton-bound amine homodimers in a water-soluble, self-assembled supramolecular host.” Proc. Nat. Acad. Sci. U.S.A.2009, 106, 10438-10443

C. J. Hastings, D. Fiedler, R. G. Bergman, K. N. Raymond. “Aza-Cope rearrangement of propargyl enammonium cations catalyzed by a self-assembled nanozyme.” J. Am. Chem. Soc.2008, 130, 10977-10983. 

A. V. Davis, D. Fiedler, M. Ziegler, A. Terpin, K. N. Raymond. “Resolution of chiral, tetrahedral M4L6 hosts.” J. Am. Chem. Soc.2007, 129, 15354-15363. 

V. M. Dong, D. Fiedler, B. Carl, R. G. Bergman, K. N. Raymond. “Molecular recognition and stabilization of iminium ions in water.” J. Am. Chem. Soc.2006, 128, 14464-14465. Highlighted in Nature2006, 444, 557. 

D. Fiedler , H. van Halbeek, R. G. Bergman, K. N. Raymond. “ Supramolecular catalysis of unimolecular rearrangements: Substrate scope and mechanistic insights.” J. Am. Chem. Soc. 2006 , 128, 10240-10252.  Highlighted in Science2006, 313, 735.

A. V. Davis, D. Fiedler, G. Seeber, A. Zahl, R. van Eldik, K. N. Raymond. “Guest exchange dynamics in an M4L6 tetrahedral host.” J. Am. Chem. Soc.2006, 128, 1324-1333. 

D. Fiedler , R. G. Bergman, K. N. Raymond. “Stabilization of reactive organometallic intermediates inside a self-assembled nanoscale host.” Angew. Chem. Int. Ed.2006, 45, 745-748. 

U. N. Andersen, G. Seeber, D. Fiedler, D. Lin, D. Harris, K. N. Raymond. “Characterization of self-assembled supramolecular host-guest complexes by electrospray ionization mass spectrometry.” J. Am. Soc. Mass. Spectrom. 2006, 17, 292-296.

D. Fiedler, D. H. Leung, R. G. Bergman, K .N. Raymond. “Selective molecular recognition, C-H Bond activation and catalysis in nanoscale reaction vessels.” Acc. Chem. Res. 2005, 38, 349-358.

D. Fiedler , R. G. Bergman, K. N. Raymond. “ Supramolecular catalysis of a unimolecular transformation: Aza-Cope rearrangement within a self-assembled host.”   Angew. Chem. Int. Ed. 200443, 6748-6751. Cover Picture. Highlighted in Angew. Chem. Int. Ed. 200544, 1000-1002.

D. Fiedler , D. H. Leung, R. G. Bergman, K. N. Raymond. “ Enantioselective guest binding and dynamic resolution of cationic ruthenium complexes by a chiral metal-ligand assembly.” J. Am. Chem. Soc. 2004,126, 3674-3675. 

D. H. Leung, D. Fiedler, R. G. Bergman, K. N. Raymond. “ Selective C-H bond activation by a supramolecular host-guest assembly.” Angew. Chem. Int. Ed200443, 963-966. 

D. Fiedler, D. Pagliero, J. L. Brumaghim, R. G. Bergman, K. N. Raymond. “ Encapsulation of cationic ruthenium complexes into a chiral self-assembled cage.” Inorg. Chem. 200443, 846-848.

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