Department Chemical Biology I (Dorothea Fiedler)

Group Publications

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2021

Daniel Couto, Annika Richter, Henriette Walter, David Furkert, Michael Hothorn, and Dorothea Fiedler. Using Biotinylated Myo -Inositol Hexakisphosphate to Investigate Inositol Pyrophosphate − Protein Interactions with Surface-Based Biosensors. ACS Chemical Biology, 2021.

Sarah Hostachy, Tillmann Utesch, Katy Franke, Gillian Leigh Dornan, David Furkert, Berke Türkaydin, Volker Haucke, Han Sun and Dorothea Fiedler. Dissecting the Activation of Insulin Degrading Enzyme by Inositol Pyrophosphates and Their Bisphosphonate Analogs. Chemical Science, 2021.

Esther Riemer, Danye Qiu, Debabrata Laha, Robert K. Harmel, Philipp Gaugler,Verena Gaugler, Michael Frei, Mohammad-Reza Hajirezaei, Nargis Parvin Laha,Lukas Krusenbaum, Robin Schneider, Adolfo Saiardi, Dorothea Fiedler, Henning J.Jessen, Gabriel Schaaf, Ricardo F.H. Giehl. ITPK1 is an InsP6/ADP phosphotransferase that controls phosphate signaling in Arabidopsis. Molecular Plant  2021.

Gangling Liao, Wenjuan Ye, Tyler Heitmann, Glen Ernst, Michael DePasquale, Laiyi Xu, Michael Wormald, Xin Hu, Marc Ferrer, Robert K. Harmel, Dorothea Fiedler, James Barrow, and Huijun Wei. "Identification of Small-Molecule Inhibitors of Human Inositol Hexakisphosphate Kinases by High-Throughput Screening." ACS Pharmacol. Transl. Sci. 2021, 4, 2, 780–789.

Martina K. Ried, Rebekka Wild, Jinsheng Zhu, Joka Pipercevic, Kristina Sturm, Larissa Broger, Robert K Harmel, Luciano A Abriata, Ludwig A Hothorn, Dorothea Fiedler, Sebastian Hiller, Michael Hothorn. Inositol pyrophosphates promote the interaction of SPX domains with the coiled-coil motif of PHR transcription factors to regulate plant phosphate homeostasis. Nat Commun. 2021.

David Furkert, Michal Nadler-Holly, Dorothea Fiedler. Affinity enrichment and identification of inositol poly- and pyrophosphate interactomes. STAR Protocols 2021.

Guangning Zong, Nikolaus Jork, Sarah Hostachy, Dorothea Fiedler, Henning J. Jessen, Stephen B. Shears, and Huanchen Wang. “New Structural Insights Reveal an Expanded Reaction Cycle for Inositol Pyrophosphate Hydrolysis by Human DIPP1.The FASEB Journal 2021. 2(35): 1-14.

Brian S. Mantilla, Karunakaran Kalesh, Nathaniel W. Brown Jr, Dorothea Fiedler, Roberto Docampo. "Affinity‐based proteomics reveals novel targets of inositol pyrophosphate (5‐IP7)‐dependent phosphorylation and binding in Trypanosoma cruzi replicative stages." Mol Microbiol. 2021; 00:1–19.

2020

Danye Qiu, Miranda S. Wilson, Verena B. Eisenbeis, Robert K. Harmel, Esther Riemer, Thomas M. Haas, Christopher Wittwer, Nikolaus Jork, Chunfang Gu, Stephen B. Shears, Gabriel Schaaf, Bernd Kammerer, Dorothea Fiedler, Adolfo Saiardi & Henning J. Jessen. "Analysis of inositol phosphate metabolism by capillary electrophoresis electrospray ionization mass spectrometry." Nat Commun 2020. 11(6035).

Alfred C. Chin, Zhe Gao, Andrew M. Riley, David Furkert, Christopher Wittwer, Amit Dutta, Tomas Rojas, Evan R. Semenza, Robin A. Felder, Jennifer L. Pluznick, Henning J. Jessen, Dorothea Fiedler, Barry V. L. Potter, Solomon H. Snyder, Chenglai Fu. "The inositol pyrophosphate 5-InsP7 drives sodium-potassium pump degradation by relieving an autoinhibitory domain of PI3K p85α." Science Advances 2020. 6(44):eabb8542

Desmarini Desmarini, Sophie Lev, David Furkert, Ben Crossett, Adolfo Saiardi, Keren Kaufman-Francis, Cecilia Li, Tania C. Sorrell, Lorna Wilkinson-White, Jacqueline Matthews, Dorothea Fiedler, Julianne Teresa Djordjevic. "IP7-SPX domain interaction controls fungal virulence by stabilizing phosphate signaling machinery." mBio 2020. 11:e01920-20

Mao Li, Robert Puschmann, Andreas Herdlitschka, Dorothea Fiedler, Helma Wennemers. "Delivery of myo‐Inositol Hexakisphosphate to the Cell Nucleus with a Proline‐Based Cell‐Penetrating Peptide." Angew. Chem. Int. Ed. 2020, 59:15586-15589

David Furkert, Sarah Hostachy, Michal Nadler-Holly, Dorothea Fiedler. "Triplexed Affinity Reagents to Sample the Mammalian Inositol Pyrophosphate Interactome." Cell Chem. Bio 2020, 27: 1-12

Soumyadip Sahu, Zhenzhen Wang, Xinfu Jiao, Chunfang Gu, Nikolaus Jork, Christopher Wittwer, Xingyao Li, Sarah Hostachy, Dorothea Fiedler, Huanchen Wang, Henning J. Jessen, Megerditch Kiledjian, Stephen B. Shears. "InsP7 is a small-molecule regulator of NUDT3-mediated mRNA decapping and processing-body dynamics." PNAS 2020, 117 (32):19245–19253.

Xingyao Li, Chunfang Gu, Sarah Hostachy, Soumyadip Sahu, Christopher Wittwer, Henning J. Jessen, Dorothea Fiedler, Huanchen Wang, Stephen B. Shears. "Control of XPR1-dependent cellular phosphate efflux by InsP8 is an exemplar for functionally-exclusive inositol pyrophosphate signaling." PNAS  2020, 117 (7):3568-3574

Robert Puschmann, Robert K. Harmel, Dorothea Fiedler. "Analysis of metabolically labeled inositol phosphate messengers by NMR." Methods Enzymol. 2020, 641:35-52

2019

Robert Puschmann, Robert K. Harmel and Dorothea Fiedler. "Scalable Chemoenzymatic Synthesis of Inositol Pyrophosphates." Biochemistry 2019, 58(38):3927-3932  ACS Editors' Choice

Jinsheng Zhu, Kelvin Lau, Robert Puschmann, Robert K Harmel, Youjun Zhang, Verena Pries, Philipp Gaugler, Larissa Broger, Amit K Dutta, Henning J Jessen, Gabriel Schaaf, Alisdair R Fernie, Ludwig A Hothorn, Dorothea Fiedler, Michael Hothorn. "Two bifunctional inositol pyrophosphate kinases/phosphatases control plant phosphate homeostasis." eLife 2019, 8:e43582

Martin Penkert, Anett Hauser, Robert Harmel, Dorothea Fiedler, Christian P. R. Hackenberger, Eberhard Krause. "Electron Transfer/Higher Energy Collisional Dissociation of Doubly Charged Peptide Ions: Identification of Labile Protein Phosphorylations" J. Am. Soc. Mass Spectrom. 2019 30(9):1578-1585

Robert K. Harmel, Robert Puschmann, Minh Nguyen Trung, Adolfo Saiardi, Peter Schmieder, Dorothea Fiedler. "Harnessing 13C-labeled myo-inositol to interrogate inositol phosphate messengers by NMR" Chem. Sci. 201910(20):5267-5274

2018

Alan M. Marmelstein, Jeremy A. M. Morgan, Martin Penkert, Daniel T. Rogerson, Eberhard Krause, Jason W Chin and Dorothea Fiedler. "Pyrophosphorylation via Selective Phosphoprotein Derivatization." Chem. Sci. 2018, 9, 5929-3596.

Cole M. Dovey, Jonathan Diep, Bradley P. Clarke, Andrew T. Hale, Dan E. McNamara, Hongyan Guo, Nathaniel W. Brown Jr., Jennifer Yinuo Cao, Christy R. Grace, Peter J. Gough, John Bertin, Scott J. Dixon, Dorothea Fiedler, Edward S. Mocarski, William J. Kaiser, Tudor Moldoveanu, John D. York, Jan E. Carette. "MLKL Requires the Inositol Phosphate Code to Execute Necroptosis." Mol Cell. 2018, 70, 936-948.

Robert Harmel & Dorothea Fiedler. "Features and regulation of non-enzymatic post-translational modifications" Nature Chemical Biology  2018, 14, 244-252

2017

Martin Penkert, Lisa M. Yates, Michael Schümann, David Perlman, Dorothea Fiedler, Eberhard Krause. "Unambiguous Identification of Serine and Threonine Pyrophosphorylation Using Neutral-Loss-Triggered Electron-Transfer/Higher-Energy Collision Dissociation." Analytical Chemistry2017, 89(6), 3672-3680

Alan M Marmelstein, Javier Moreno, Dorothea Fiedler. "Chemical Approaches to Studying Labile Amino Acid Phosphorylation." Top Curr Chem (J)2017, 375(2):22

2016

Mingxuan Wu, Lucy S. Chong, David H. Perlman, Adam C. Resnick, Dorothea Fiedler. "Inositol Polyphosphates intersect with signalling and metabolic networks via two distinct mechanisms." PNAS2016, 113(44), E6757-6765

Manasa Chanduri, Ashim Rai, Aushaq Bashir Malla, Mingxuan Wu, Dorothea Fiedler, Roop Mallik, Rashna Bhandari. "Inositol hexakisphosphate kinase 1 (IP6K1) activity is required for cytoplasmic dynein-driven transport." Biochemical Journal2016, 473(19) 3031-304

Anastasia Hager, Mingxuan Wu, Huanchen Wang, Nathaniel W. Brown Jr., Stephen B Shears, NicolásVeiga, Dorothea Fiedler. "Cellular Cations Control Conformational Switching of Inositol Pyrophosphate Analogues." Chem. Eur. J.2016, 22, 12406-12414 (Hot Paper)

Nathaniel W. Brown, Alan M. Marmelstein, Dorothea Fiedler. "Chemical tools for interrogating inositol pyrophosphate structure and function." Chem. Soc. Rev.2016, 45, 6311-26

Lisa M. Yates and Dorothea Fiedler. "A Stable Pyrophosphoserin Analog for Incorporation into Peptides and Proteins." ACS Chem Biol.2016, 11(4), 1066-1073

Elizabeth A. Steidle, Lucy S. Chong, Mingxuan Wu, Elliot Crooke, Dorothea Fielder, Adam C. Resnick, Ronda J. Rolfes. "A novel inostiol pyrophosphate phosphatase in Saccharomyces cerevisiae: Siw14 selectively cleaves the β-phosphate from 2-diphosphoinositol pentakisphosphate (5PP-IP5)" J. Biol. Chem.2016, 291(13), 6772-83

2015

Florence J. Williams and Dorothea Fiedler. "A Fluorescent Sensor and Gel Stain for Detection of Pyrophosphorylated Proteins" ACS Chem. Biol.2015, 10(9), pp 1958-1963

John H. Conway and Dorothea Fiedler. "An Affinity Reagent for the Recognition of Pyrophosphorylated Peptides" Angew. Chem. Int. Ed.2015, 54, 3941-3945.

Lisa M. Yates and Dorothea Fiedler. "Establishing the Stability and Reversability of Protein Pyrophosphorylation with Synthetic Peptides" ChemBioChem2015, 16, 415-423. (Cover Picture, Highlighted in ChemViews Magazine: Lost in Post-Translation)

2014

Lisa M. Yates, Alan M. Marmelstein, and Dorothea Fiedler. "Synthetic Pyrophosphorylation Methods and Their Use in Chemical Biology." Synlett2014, 25, 2239-2245.

Mingxuan Wu, Lucy S. Chong, Samanta Capolicchio, Henning J. Jessen, Adam C. Resnick, and Dorothea Fiedler. "Elucidating Diphosphoinositol Polyphosphate Function with Nonhydrolyzable Analogues" Angew. Chem. Int. Ed. 2014, 53, 7192-7197.

Feng Roa, Jiyoung Cha, Jing Xu, Risheng Xu, M. Scott Vandiver, Richa Tyagi, Robert Tokhunts, Michael A. Koldobskiy, Chenglai Fu, Roxanne Barrow, Mingxuan Wu, Dorothea Fiedler, James C. Barrow, Solomon H. Snyder. "Inositol Pyrophosphates Mediate the DNA-PK/ATM-p53 Cell Death Pathway by Regulating CK2 Phosphorylation of Tti1/Tti2." Mol. Cell2014, 54, 119-132.

Alan M. Marmelstein, Lisa M. Yates, John H. Conway, and Dorothea Fiedler. "Chemical Pyrophosphorylation of Functionally Diverse Peptides." J. Am. Chem. Soc. 2014, 136, 108-111. (Highlighted in JACS Spotlights: J. Am. Chem. Soc. 2014, 136, 108-111.)

2013

Mingxuan Wu, Barbara E. Dul, Alexandra J. Trevisan, and Dorothea Fiedler. "Synthesis and characterization of non-hydrolysable diphosphoinositol polyphosphate messengers." Chem. Sci.2013, 4, 405-410.

Previous Publications

Postdoctoral

J. I. Kliegman, D. Fiedler, C. J. Ryan, Y. F. Xu, X. Y. Su, D. Thomas, M. C. Caccese, A. Cheng, M. Shales, J. D. Rabinowitz, N. J. Krogan, K. M. Shokat. " Chemical Genetics of Rapamycin-Insensitive TORC2 in S. cerevisiae." Cell Reports 2013, 5, 1725–1736.

J. L. Nakamura, C. Phong, E. Pinarbasi, S. C. Kogan, S. Vandenberg, A. E. Horvai, B. A. Faddegon, D. Fiedler, K. M. Shokat, B. T. Houseman, R. Chao, R. O. Pieper, K. Shannon. “Dose-dependent effects of focal fractionated irradiation on secondary malignant neoplasms in Nf1 mutant mice.” Cancer Res.2011, 71, 106-15.

S. van Wageningen, P. Kemmeren, P. Lijnzaad, T. Margaritis, J. J. Benschop, I. J. de Castro, D. van Leenen, M. J. A. Groot Koerkamp, C. W. Ko, A. J. Miles, N. Brabers, M.   O. Brok, T. L. Lenstra, D. Fiedler, L. Fokkens, R. Aldecoa, E. Apweiler, V. Taliadouros, K. Sameith, L. A. L. van de Pasch, S. R. van Hooff, L. V. Bakker, N. J. Krogan, B. Snel, F.   C. P. Holstege. “Functional overlap and regulatory links shape genetic interactions between signaling pathways.” Cell2010, 143, 991-1004. Highlighted in Cell2010, 143, 867-869. 

S. Bandyopadhyay, M. Mehta, D. Kuo, M. K. Sung, R. Chuang, E. J. Jaehnig, B> Bodenmiller, K. Licon, W. Copeland, M. Shales, D. Fiedler, J. Dutkowski, A. Guénolé, H. van Attikum, K. M. Shokat, R. D. Kolodner, W. K. Huh, R. Aebersold, M. C. Keogh, N. J. Krogan, T. Ideker. “Rewiring of genetic networks in response to DNA damage.” Science2010, 330, 1385-1389.

P. Beltrao, J. C. Trinidad, D. Fiedler, A. Roguev, W. A. Lim, K. M. Shokat, A. L. Burlingame, N. J. Krogan. “Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.” PLoS Biol.2009, 7, e1000134.

D. Fiedler , H. Braberg, M. Mehta, G. Chechik, G. Cagney, P. Mukherjee, A. C. Silva, M. Shales, S. R. Collins S. van Wageningen, P. Kemmeren, F. C. P. Holstege, J. S. Weissman, M. Christopher-Keogh, D. Koller, K. M. Shokat, N. J. Krogan. “Functional organization of the S. cerevisiae phosphorylation network.” Cell 2009136, 952- 963.  Cover Picture

T. Okuzumi, D. Fiedler, C. Zhang, D. C. Gray, B. Aizenstein, R. Hoffman, K. M. Shokat. “Inhibitor hijacking of Akt activation.” Nat. Chem. Bio. 20095, 484-493.

H. Wang, B. Kakaradov, S. R. Collins, L. Karotki, D. Fiedler, M. Shales, K. M. Shokat, T. Walther, N. J. Krogan, D. Koller. “A complex-based reconstruction of the S. cerevisiae interactome.“ Mol. Cell. Proteomics 20098, 1361-1381.

L. M. Sly; M. J. Hamilton; E. Kuroda; V. W. Ho; F. L. Antignano; S. L. Omeis; C. J. van Netten-Thomas; D. Wong; H. K. Brugger; O. Williams; M. E. Feldman; B. T. Houseman; D. Fiedler; K. M. Shokat; G. Krystal. “ SHIP prevents lipopolysaccharide from triggering an antiviral response in mice.” Blood 2009113, 2945-2954.

J. Wang, Z. Knight, D. Fiedler, O. R. Williams, K. M. Shokat, D. Pearce. “Activity of the p110-alpha subunit of phosphatidylinositol-3-kinase is required for activation of epithelial sodium transport.” Am. J. Physiol. 2008295, F843-F850. 

T. Tamguney, C. Zhang, D. Fiedler, K. M. Shokat, D. Stokoe. “Analysis of 3-phosphoinositide-dependent kinase-1 signaling and function in ES Cells.” Exp. Cell Res. 2008314, 2299-2312.

Graduate

J. S. Mugridge, D. Fiedler, K. N. Raymond. “A Ferrocene-Based Catecholamide Ligand: the Consequences of Ligand Swivel for Directed Supramolecular Self-Assembly.” J. Coord. Chem.2010, 63, 2779 – 2789.

M. Pluth, D. Fiedler, R. G. Bergman, K. N. Raymond. “Molecular recognition and self-assembly special feature: Encapsulation and characterization of proton-bound amine homodimers in a water-soluble, self-assembled supramolecular host.” Proc. Nat. Acad. Sci. U.S.A.2009, 106, 10438-10443

C. J. Hastings, D. Fiedler, R. G. Bergman, K. N. Raymond. “Aza-Cope rearrangement of propargyl enammonium cations catalyzed by a self-assembled nanozyme.” J. Am. Chem. Soc.2008, 130, 10977-10983. 

A. V. Davis, D. Fiedler, M. Ziegler, A. Terpin, K. N. Raymond. “Resolution of chiral, tetrahedral M4L6 hosts.” J. Am. Chem. Soc.2007, 129, 15354-15363. 

V. M. Dong, D. Fiedler, B. Carl, R. G. Bergman, K. N. Raymond. “Molecular recognition and stabilization of iminium ions in water.” J. Am. Chem. Soc.2006, 128, 14464-14465. Highlighted in Nature2006, 444, 557. 

D. Fiedler , H. van Halbeek, R. G. Bergman, K. N. Raymond. “ Supramolecular catalysis of unimolecular rearrangements: Substrate scope and mechanistic insights.” J. Am. Chem. Soc. 2006 , 128, 10240-10252.  Highlighted in Science2006, 313, 735.

A. V. Davis, D. Fiedler, G. Seeber, A. Zahl, R. van Eldik, K. N. Raymond. “Guest exchange dynamics in an M4L6 tetrahedral host.” J. Am. Chem. Soc.2006, 128, 1324-1333. 

D. Fiedler , R. G. Bergman, K. N. Raymond. “Stabilization of reactive organometallic intermediates inside a self-assembled nanoscale host.” Angew. Chem. Int. Ed.2006, 45, 745-748. 

U. N. Andersen, G. Seeber, D. Fiedler, D. Lin, D. Harris, K. N. Raymond. “Characterization of self-assembled supramolecular host-guest complexes by electrospray ionization mass spectrometry.” J. Am. Soc. Mass. Spectrom. 2006, 17, 292-296.

D. Fiedler, D. H. Leung, R. G. Bergman, K .N. Raymond. “Selective molecular recognition, C-H Bond activation and catalysis in nanoscale reaction vessels.” Acc. Chem. Res. 2005, 38, 349-358.

D. Fiedler , R. G. Bergman, K. N. Raymond. “ Supramolecular catalysis of a unimolecular transformation: Aza-Cope rearrangement within a self-assembled host.”   Angew. Chem. Int. Ed. 200443, 6748-6751. Cover Picture. Highlighted in Angew. Chem. Int. Ed. 200544, 1000-1002.

D. Fiedler , D. H. Leung, R. G. Bergman, K. N. Raymond. “ Enantioselective guest binding and dynamic resolution of cationic ruthenium complexes by a chiral metal-ligand assembly.” J. Am. Chem. Soc. 2004,126, 3674-3675. 

D. H. Leung, D. Fiedler, R. G. Bergman, K. N. Raymond. “ Selective C-H bond activation by a supramolecular host-guest assembly.” Angew. Chem. Int. Ed200443, 963-966. 

D. Fiedler, D. Pagliero, J. L. Brumaghim, R. G. Bergman, K. N. Raymond. “ Encapsulation of cationic ruthenium complexes into a chiral self-assembled cage.” Inorg. Chem. 200443, 846-848.

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