Cell Engineering (Ralf Schülein)

Order page

For an order, we need some information. To get the order form below as a word file, please write an E-Mail to Ralf Schülein schuelein@fmp-berlin.de

1. General
- Date:
- Orderer (Name/Phone/Email)
- Group leader:
We assume that the group leader has approved this order

2. Informations about your CRISPR/Cas project
- Gene of interest:
- NCBI reference sequence
- UniProt accession number:
- Please provide a file describing intron/exon organization (SnapGene or equivalent):
- Type of gene editing, knock-out or knock-in:
- In case of knock-in, please specify:
- Cell type:
(we prefer to use our own cells; in case your cells are needed, we will first make a test for  mycoplasms prior of including your cells into the facility)
- Specific antibody available?

3. Genetic engineering, Biosafety
- LAGeSo approval number:
- Safety level:

How your order will be processed by the Cell Engineering facility
- Primer design and prediction of potential off-target sites
- Your approval of potential off-target sites
- Gene editing
- Selection of cell clones
- Confirmation of gene editing by Western blotting and/or DNA sequencing
- Genotyping

Depending on the amount of simultaneous orders, clones should be ready within 8-12 weeks. Depending on our expenses and the amount of sgRNAs needed, the costs for a cell clone will be in range of 1,500 to 2,500€ and will be charged once the clones are ready.  For reasons of capacity, we are not able to analyze potential off-target effects experimentally.

 

Leibniz-Forschungsinstitut für Molekulare Pharmakologie im Forschungsverbund Berlin e.V. (FMP)
Campus Berlin-Buch
Robert-Roessle-Str. 10
13125 Berlin, Germany
+4930 94793 - 100 
+4930 94793 - 109 (Fax)
info(at)fmp-berlin.de

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