2020

Gallrein C, Iburg M, Michelberger T, Koçak A, Puchkov D, Liu F, Ayala Mariscal SM, Nayak T, Kaminski Schierle GS & Kirstein J: Novel Amyloid-beta pathology C. elegans model reveals distinct neurons as seeds of pathogenicity

Progress in Neurobiology, in press

Haenig C, Atias N,  Taylor AK, Mazza A, Schaefer MH, Russ J, Riechers SP, Jain S, Coughlin M, Fontaine JF, Freibaum BD, Brusendorf L, Zenkner M, Stroedicke M, Schnoegl S, Arnsburg K, Boeddrich A, Pigazzini ML, Heutink P, Taylor JP, Kirstein J, Andrade-Navarro MA, Sharan R, Wanker EE

Interactome mapping provides a network of neurodegenerative disease proteins and uncovers widespread protein aggregation in affected brains

Cell Reports, 2020 Aug 18;32(7):108050. doi: 10.1016/j.celrep.2020.108050.

Claes F, Rudyak S, Laird AS, Louros N, Beerten J, Debulpaep M, Michiels E, van der Kant R, Van Durme J, De Baets G, Houben B, Ramakers M, Yuan K, Gwee SSL, Hernandez S, Broersen K, Oliveberg M, Moahamed B, Kirstein J, Robberecht W, Rousseau F, Schymkowitz J 

Exposure of a cryptic Hsp70 binding site determines the cytotoxicity of the ALS-associated SOD1-mutant A4V

Protein Engineering, Design and Selection, 2019 Dec 31;32(10):443-457. doi: 10.1093/protein/gzaa008.

Pigazzini ML & Kirstein J

In vivo quantification of protein turnover in aging C. elegans using the photoconvertable Dendra2

J. Vis Exp., 2020 Jun 13;(160). doi: 10.3791/61196.

Iburg M, Puchkov D, Brugada IUR, Bergemann L, Rieprecht U & Kirstein J

The non-canonical small heat shock protein HSP-17 from C. elegans is a selective protein aggregase

J Biol Chem, Jan 30 doi: 10.1074/jbc.RA119.011185.

Pigazzini ML, Gallrein C, Iburg M, Kaminski Schierle G, Kirstein J

Characterisation of amyloid structures in aging C. elegans using fluorescence lifetime imaging. 

J. Vis Exp., 2020 Mar 27;(157). doi: 10.3791/61004

2019

Sandhof CA, Hoppe SO, Druffel-Augustin S, Gallrein C, Kirstein J, Voisine C, Nussbaum-Krammer C

Reducing INS-IGF1 Signaling Protects Against Non-Cell Autonomous Vesicle Rupture Caused by SNCA Spreading

Autophagy, 2019 Jul 29:1-22.

Feleciano DR, Juenemann K, Iburg M, Bras I, Holmberg CI, Kirstein J

Crosstalk between chaperone-mediated protein disaggregation and proteolytic pathways in aging and disease”

Frontiers in Aging Neuroscience, 2019 Jan 29;11:9

Kreis P*#, Gallrein C#, Rojas-Puente E#, Mack TGA, Kroon C, Dinkel V, Willmes C, Murk K, tom-Dieck S, Schuman EM, Kirstein J*, Eickholt BJ*

ATM phosphorylation of the actin-binding protein drebrin controls oxidation stress-resistance in mammalian neurons and C. elegans

Nature Communications, 019 Jan 30;10(1):486 (# co-first authors; * co-corresponding authors)

2018

Kampinga HH, Andreasson C, Barducci A, Cheetham ME, Cyr D, Emanuelsson C, Genevaux P, Gestwicki JP,  Goloubinoff P, Huerta-Cepas J, Kirstein J, Liberek K, Mayer MP, Nagata K, Nillegoda NB, Pulido P, Ramos C, De los Rios P, Rospert S,  Rosenzweig R, Sahi C, Taipale M, Tomiczek B, Ushioda R, Young JC, Zimmermann R, Zylicz A, Zylicz M, Craig EA, Marszalek J

Function, evolution, and structure of J-domain proteins

Cell Stress and Chaperones, 2018 Nov 26. doi: 10.1007/s12192-018-0948-4

Ast A, Buntru A, Schindler F, Hasenkopf R, Schulz A, Brusendorf L, Klockmeier K, Grelle G, McMahon B, Niederlechner H, Jansen I, Diez LM, Boeddrich A, Franklin SA, Baldo B, Schnoegl S, Kunz S, Purfürst B, Kampinga HH, Morton AJ, Petersén A, Kirstein J, Bates GP, Wanker EE

Mutant HTT seeding activity: a marker of disease progression and neurotoxicity in models of Huntington’s disease.

Molecular Cell, 2018; 71(5):675-688

Juenemann K, Jansen AHP, van Riel L, Merkx R, Mulder MPC, An H, Statsyuk A, Kirstein J, Ovaa H, Reits EA

Dynamic recruitment of ubiquitin to mutant huntingtin inclusion bodies

Scientific Reports, 2018 23;8(1):1405

Scior A, Buntru A, Arnsburg K, Ast A, Iburg M, Juenemann K, Pigazzini ML, Mlody B, Puchkov D, Priller J, Wanker EE, Prigione A, Kirstein J

Complete suppression of Htt fibrilization and disaggregation of Htt fibrils by a chaperone complex. 

The EMBO Journal, 2018; 37(2)282-299

2017

Kirstein J, Arnsburg K, Scior A, Szlachcic A, Guilbride DL, Morimoto RI, Bukau B & Nillegoda NB

In vivo properties of the disaggregase function of J-proteins and Hsc70 in C. elegans stress and aging

Aging Cell, 2017 Oct 10. doi: 10.1111/acel.12686.

Lechler MC, Crawford ED, Groh N, Widmaier K, Jung R, Kirstein J, Trinidad JC, Burlingame AL, David DC

Reduced Insulin/IGF-1 Signaling Restores the Dynamic Properties of Key Stress Granule Proteins during Aging

Cell Reports, 2017

2016

Feleciano RD, Arnsburg K & Kirstein J

Interplay between redox and protein homeostasis

Worm, 2016

Scior A, Juenemann K & Kirstein J

Cellular strategies to cope with protein aggregation

Essays in Biochemistry, 2016

2015

Feleciano RD & Kirstein J

Collapse of redox homeostasis during aging and stress

Molecular & Cellular Oncology, 2015

Nillegoda N, Kirstein J, Szlachcic A, Berynskyy M, Stank A, Stengel F, Arnsburg K, Gao X, Scior A, Aebersold R, Guilbride DL, Wade RC, Morimoto RI, Mayer MP and Bukau B

Crucial Hsp70 co-chaperone complex unlocks metazoan protein disaggregation

Nature, 2015 

Kirstein J*, Morito D, Kakihana T, Sugihara M, Minnen A, Hipp MS, Nussbaum-Krammer C, Hartl FU*, Nagata K* & Morimoto RI* 

Proteotoxic stress and ageing triggers the loss of redox homeostasis across cellular compartments

EMBO J, 2015  (* corresponding authors)

2014

Arnsburg, K & Kirstein-Miles, J

Interrelation between Protein Synthesis, Proteostasis and Life Span

Current Genomics, 2014

Kirstein-Miles J, Morimoto RI

Protein Homeostasis in Health and Aging

Molecular and Cellular Biology of Aging

Bartlett and Jones Publishers, 2014

2013

Kirstein-Miles J, Morimoto RI

Ribosome-associated chaperones act as proteostasis sentinels.

Cell Cycle, 2013

Kirstein-Miles J*, Scior A*, Deuerling E, Morimoto RI

The nascent polypeptide associated complex is a key regulator of proteostasis.

EMBO J, 2013; (* equal contribution) 

2012

Rampelt H, Kirstein-Miles J, Nillegoda NB, Kang C, Scholz SR, Morimoto RI, Bukau B 

Metazoan Hsp70 machines use Hsp110 to power protein disaggregation.

EMBO J, 2012 

Lowth BR, Kirstein-Miles J, Broetz-Oesterhelt H, Morimoto RI, Truscott KN, Dougan DA

Identification and characterization of hClpX-interacting proteins, using a hClpXP Walker B mutant. 

J Struct. Biol, 2012 

2010

Wolf D, Kalamorz F, Wecke T, Juszczak A, Mäder U, Homuth G, Jordan S, Kirstein J, Hoppert M, Voigt B, Hecker M, Mascher T.

In-depth profiling of the LiaR response of Bacillus subtilis.

J Bacteriol, 2010 

Kirstein-Miles J, Morimoto RI

Peptides signal mitochondrial stress.

Cell Metab, 2010 

Kirstein-Miles J, Morimoto RI

Caenorhabditis elegans as a model system to study intercompartmental proteostasis: Interrelation of mitochondrial function, longevity, and neurodegenerative diseases.

Dev Dyn, 2010

2009

Kirstein J, Moliere N, Dougan DA, Turgay K

Adapting the machine: Adaptor proteins for Hsp100/Clp and AAA+ proteases

Nat Rev Microbiol, 2009

Kirstein J, Hoffmann A, Lilie H, Schmidt R, Ruebsamen-Waigmann H, Broetz-Oesterhelt H, Mogk A, Turgay K

The antibiotic ADEP reprograms ClpP, switching it from a regulated to an uncontrolled protease. 

EMBO Mol Med, 2009 

2008

Kirstein J, Strahl H, Moliere N, Hamoen, L & Turgay K

Localization of general and regulatory proteolysis in Bacillus subtilis cells.

Mol Microbiol, 2008 

Haslberger T, Zdanowicz A, Brand I, Kirstein J, Turgay K, Mogk A, Bukau B

Protein disaggregation by the AAA+ chaperone ClpB involves partial threading of looped polypeptide segments.

Nat Struct Mol Biol, 2008 

Erbse AH, Wagner JN, Truscott KN, Spall SK, Kirstein J, Zeth K, Turgay K, Mogk A, Bukau B, Dougan DA

Conserved residues in the N-domain of the AAA+ chaperone ClpA regulate substrate recognition and unfolding 

FEBS J, 2008 

2007

Kirstein J, Dougan DA, Gerth U, Hecker M & Turgay K

The tyrosine kinase McsB is a regulated adaptor protein for ClpCP

EMBO J, 2007

2006

Kirstein J, Schlothauer T, Dougan DA, Lilie H, Tischendorf G, Mogk A, Bukau B, Turgay K.

Adaptor protein controlled oligomerization activates the AAA+ protein ClpC.

EMBO J, 2006 

Andersson FI, Blakytny R, Kirstein J, Turgay K, Bukau B, Mogk A, Clarke AK.

Cyanobacterial ClpC/HSP100 protein displays intrinsic chaperone activity.

J Biol Chem, 2006 

2005

Kirstein J, Zuhlke D, Gerth U, Turgay K, Hecker M.

A tyrosine kinase and its activator control the activity of the CtsR heat shock repressor in B. subtilis.

EMBO J, 2005 

Kirstein J, Turgay K.

A new tyrosine phosphorylation mechanism involved in signal transduction in Bacillus subtilis.

J Mol Microbiol Biotechnol, 2005

2004

Gerth U, Kirstein J, Mostertz J, Waldminghaus T, Miethke M, Kock H, Hecker M.

Fine-tuning in regulation of Clp protein content in Bacillus subtilis.

J Bacteriol, 2004 

2003

Rollenhagen C, Antelmann H, Kirstein J, Delumeau O, Hecker M, Yudkin MD.

Binding of sigma(A) and sigma(B) to core RNA polymerase after environmental stress in Bacillus subtilis.

J Bacteriol, 2003