Department Physiology and Pathology of Ion Transport (Thomas J. Jentsch)

M.Sc. THESIS - currently open position ! (08/2018)

A Master thesis position is vacant in the Jentsch lab: The project offers the student the possibility to gather experience with cloning techniques, confocal microscopy and cell culture. There are different options to choose from: e.g. production of CRISPR/Cas9 KI and KO cell lines with subsequent phenotypic characterization by use of fluorescence microscopy // studying the role of the chloride channel ClC-7 in cellular ion homeostasis and signalling with biochemistry and lipid-protein interaction assays.

Enthusiastic and motivated students are welcome to apply and do a 6 months research internship for the M.Sc. project in a first class and at the same time friendly and stimulating environment.

Further details will be discussed in a personal colloquium. Please direct your application with CV to Carlo.Barbini@mdc-berlin.de

Postdoctoral and doctoral student positions in the group 'Physiology and Pathology of Ion Transport" (Thomas J. Jentsch)

in the areas of

Cell Biology, Biophysics (Electrophysiology and/or Ion Imaging), Molecular Physiology and Morphology

We are an interdisciplinary team working on diverse aspects of ion transport, with a focus on chloride and potassium channels and on KCl cotransport. We are interested in their molecular biology and structure and function (analysed by mutagenesis and electrophysiology, including patch-clamp), their involvement in human diseases (we have elucidated the mechanisms of myotonia congenita, Dent’s disease (a renal disease associated with proteinuria and kidney stones), severe renal salt loss, neonatal epilepsy,  DFNA2 (a dominant deafness), osteopetrosis, ánd lysosomal storage disease), and their cell biology (e.g. involvement in endocytosis and intravesicular acidification).

We also use knock-out mouse models to unravel their physiological functions. A major focus of our present research is the role of intracellular CLC chloride channels, for which we have generated several knock-out mouse models. These channels are involved in acidifying intracellular compartments (e.g. endosomes and synaptic vesicles). This acidification is crucial for many receptor-ligand interactions, enyzmatic activities, and trafficking of vesicles and membrane proteins. Accordingly, these knock-outs have yielded highly interesting and in part surprising results: disruption of ClC-5 leads to a broad defect in endocytosis, disruption of ClC-3 to neurodegeneration, and the loss of ClC-7 to osteopetrosis and neurodegeneration. Recent surprising results indicate that vesicular ClCs are Cl/H exchangers and not Cl-channels. These studies have opened a new avenue in the research of intracellular trafficking and sorting events.

Another focus of our research is on the role of KCNQ potassium channels in the nervous system. We have previously shown that KCNQ2 is mutated in a form of human epilepsy, and KCNQ4 in a form of human deafness. We have generated several KCNQ mouse models which are used to study the roles of KCNQ channels in the CNS and PNS

Recently, we have expanded our research to include Ca-activated chloride channels of the anoctamin gene family and have just published a paper on the role of Ano2 in olfaction.

Recent publications include:  EMBO J, 24: 1079 (2005); Nature 436: 424 (2005); EMBO J 25: 642 (2006);  Nature 440: 220 (2006); EMBO J 27:2907 (2008). FASEB J 23: 4056 (2009); Science 328: 1398 (2010); Science 328: 1401 (2010); Nature Neurosci 14: 763 (2011); Nature Neurosci 15: 138 (2012).

POSTDOCs 

We are looking for postdocs with expertise in Cell Biology or Biophysics (Electrophysiology and/or Ion imaging).

For the position in Electrophysiology and/or Ion Imaging, prior experience in patch-clamping, in particular slice physiology, would be an asset. Our knock-out mouse models, e.g. of synaptic vesicle chloride channels or KCl cotransport, lead to many fascinating problems that can be tackled by an electrophysiological analysis of slice preparations or cultured cells derived from these mice. Experience with exocytosis and endocytosis (e.g. capacitance measurements) would also be valuable.
In the Ion Imaging field, we would in particular like to study the luminal pH and chloride of vesicles, but also use e.g. Ca-imaging as an indicator of neuronal activity. For this focus of research, prior experience in ion imaging would be important. In addition, applications of biophysicists interested in structure-function relationships of cloned channels are also welcome.
Our lab is well-equipped in this area with five patch-clamp set-ups, two 2-electrode voltage-clamp set-ups for oocytes,  two ion imaging set-ups, TIRF and confocal microscopes. Applications of physicists are particularly welcome.

For the position in Cell Biology, previous experience in intracellular trafficking, cell fractionation, vesicle fusion, sorting, or biochemistry of membrane proteins is desirable. Our research on intracellular chloride channels and their role in trafficking, including the generation of several highly relevant KO mouse models, has given us the tools to tackle vesicle trafficking and function from a very new perspective.

DOCTORAL STUDENTS 

For these positions, we are either looking for students interested in Cell Biology, Neurobiology and Electrophysiology (which will be combined with molecular biology at later stages), or for students interested in Neurobiology, Cell Biology, Molecular Biology, Physiology, and Morphology. Prior experience in one of these areas would be advantageous, but is not necessary. Applications of biochemists, biologists, chemists, and physicists are welcome. Doctoral students may join the GRADUATE PROGRAM of either the FMP or MDC.

Please direct your applications with full CV and references to:

Prof. Thomas J. Jentsch, FMP/MDC
Robert-Rössle-Str. 10
13125 Berlin
Tel: 030 9406 2961
Fax: 030 9406 2960
e-mail: jentsch@fmp-berlin.de.

For more detailed information see our research summaries and the homepage of our group.

Leibniz-Forschungsinstitut für Molekulare Pharmakologie im Forschungsverbund Berlin e.V. (FMP)
Campus Berlin-Buch
Robert-Roessle-Str. 10
13125 Berlin, Germany
+4930 94793 - 100 
+4930 94793 - 109 (Fax)
info(at)fmp-berlin.de

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