Department of NMR-Supported Structural Biology
(Hartmut Oschkinat)
Prof. Dr. Hartmut Oschkinat
Leibniz-Institut für Molekulare Pharmakologie (FMP)
Department NMR-supported Structural Biology
Robert-Rössle-Str. 10 / D 13125 Berlin
Phone: +49 (0)30 / 94793 160
Fax: +49 (0)30 / 94793 169
E-mail: oschkinat@fmp-berlin.de
Research Interests and Achievements
Structural characterisation of protein-protein interactions responsible for the reception and transduction of signals in biological systems, and their disruption by small molecules. Protein domains which recognize peptides with characteristic motifs, proteins involved in protein homeosthasis, structural investigations on membrane proteins and cytoskeleton-attached proteins by solid state NMR.
More than 180 publications in the fields of protein structure and NMR methods development. More than 50 structures deposited in the PDB. Among those are first structures of the PH- and WW-domains. Development of small molecular inhibitors of interactions of PDZ domains. First assignment of protein resonances on the basis of solid-state NMR data, first structure of a protein by solid-state NMR. Structure of a fibril formed by a WW-domain at atomic resolution. Structure of alphaB-crystallin oligomers by solid-state NMR and SAXS. Applications of dynamic nuclear polarisation to large biological complexes. Structure of the dark-adapted form of bacteriorhodopsin by solution NMR.
Curriculum Vitae
2009-2011 | Acting Director of the Leibniz-Institut für Molekulare Pharmakology (FMP), Berlin | |
| Since 1998 | Head of the department "NMR-supported Structural Biology“ at the Leibniz-Institut für Molekulare Pharmakologie, Professor of Structural Chemistry at the Free University in Berlin | |
| 1992-1998 | Group leader at the EMBL, Heidelberg. Work on structure and function of signalling domains | |
| 1992 | Habilitation in Biophysical Chemistry at the Technical University of Munich | |
| 1987-1991 | Position as NMR-spectroscopist at the Max-Planck-Institute for Biochemistry (Martinsried, Germany), first in the Clore/Gronenborn group, later independently in the department of Prof. Huber. Pulse sequence development, 3D-NMR | |
| 1986-1987 | Postdoctoral work with Prof. Dr. Bodenhausen at the University of Lausanne, Switzerland; work on pulse sequences | |
| 1986 | Graduate thesis: "Analysis of the conformation of Cyclosporin in solution using NMR-spectroscopy: development and use of new methods" | |
| 1983-1985 | Completion of dissertation in Prof. Kessler's Laboratory at the University of Frankfurt | |
| 1983-1984 | Visit to the laboratory of Prof. Dr. Ray Freeman, Oxford, England | |
| 1975-1976 and 1978-1983 | Chemistry degree at the University of Frankfurt, with merit; Diploma (excellent) | |
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Activities in the scientific community
1998 EMBO-Membership
Editorial Boards
Journal of Biomolecular NMR, Springer Netherlands
Structure, Elsevier, San Diego
Journal of Structural Genomics, Springer Netherlands
Advisory Boards
CEITEC, Brno, Czech Republic
CCPN, Cambridge, UK
Selected Publications
Jehle S, Rajagopal P, Bardiaux B, Markovic S, Kühne R, Stout JR. Higman VA, Klevit RE, van Rossum BJ, Oschkinat H (2010) Solid-state NMR and SAXS studies provide a structural basis for the activation of aB-crystallin oligomers. Nat Struct Mol Biol, 17: 1037-1042.
Krabben L, van Rossum BJ, Jehle S, Bocharov E, Lyukmanova EN, Schulga AA, Arseniev A, Hucho F, Oschkinat H (2009) Loop 3 of short neurotoxin II is an additional interaction site with membrane-bound nicotinic acetylcholine receptor as detected by solid-state NMR spectroscopy. J Mol Biol, 390:662-671.
Pellecchia M, Bertini I, Cowburn D, Dalvit C, Giralt E, Jahnke W, James TL, Homans SW, Kessler H, Luchinat C, Meyer B, Oschkinat H, Peng J, Schwalbe H, Siegal G (2008) Perspectives on NMR in drug discovery: a technique comes of age. Nat Rev Drug Discov, 7(9):738-745.
Ferguson N, Becker J, Tidow H, Tremmel S, Sharpe TD, Krause G, Flinders J, Petrovich M, Berriman J, Oschkinat H, Fersht AR (2006) General structural motifs of amyloid protofilaments. Proc Natl Acad Sci USA 103:16248-16253.
Joshi M, Vargas C, Boisguerin P, Diehl A, Krause G, Schmieder P, Moelling K, Hagen V, Schade M, Oschkinat H. 2006. Discovery of low-molecular-weight ligands for the AF6 PDZ domain. Angew Chem Int Ed Engl. 45(23):3790-3795.
Castellani F, van Rossum BJ, Diehl A, Schubert M, Rehbein K, Oschkinat H (2002) Structure of a protein determined by solid-state magic-angle-spinning NMR spectroscopy. Nature 420:98-102.
Macias MJ, Gervais V, Civera C, Oschkinat H (2000) Structural analysis of WW domains and design of a WW prototype. Nat Struc Biol 7:375-79.
Macias MJ, Hyvˆnen M, Baraldi E, Schultz J, Sudol M, Saraste M, Oschkinat H (1996) Structure of the WW domain of a kinase-associated protein complexed with a proline-rich peptide. Nature 382:646-9.
Macias-Hernandez M, Musacchio A, Ponstingl H, Nilges M, Saraste M, Oschkinat H (1994) Structure of the pleckstrin homology domain from b-spectrin. Nature 369:675-7.
Oschkinat H, Griesinger C, Kraulis PJ, SÀrensen OW, Ernst RR, Gronenborn AM, Clore GM (1988) Three-dimensional NMR-spectroscopy of a protein in solution. Nature 332:374-6.
